用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
采用细胞器-细胞荧光强度比值法，对HMME进行单细胞内分布的定性与定量研究。
Organelle-cell fluorescence intensity ratio analysis was adopted to study the intracellular distribution of HMME.
实际用于混合物荧光定性定量分析中，发现它比广义减秩法（GRA）结果稳定、可靠。
It has been used practically in qualitative and quantitative analysis of fluorescent mixtures and a comparison was made with generalized rank annihilation method (GRA).
介绍了用XRF（X射线荧光光谱法）测定锆英石质耐火材料中的锆含量的定量分析方法。
The quantitative analysis is described for the zirconium content by use of XRF(X-ray fluorescence method) zircon stone refractory in this paper.
用原子吸收光谱法、原子荧光光谱法和等离子发射光谱法，定性检测颗粒物中载带的重金属，并对其中9种重金属进行定量测定。
The heavy metals were detected with atomic absorption spectrum, atomic fluorescence spectrum and plasma emission spectrum, 9 heavy metals were measured quantitatively.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法痰中病原菌定量培养、鉴定及荧光免疫法检测acb及其抗体类型。
METHODS Quantitative culture of pathogenic organisms in sputum samples and immunofluorescence method were used to detect ACBs and their types.
了解小儿乙型肝炎病毒(HBVDNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM，荧光定量pcr法检h BV DNA。
To investigate the change of HBV DNA in children with hepatitis B. HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.
结论荧光定量PCR法在检测沙眼体较金标法更敏感、快速，是早期诊断生殖道沙眼衣原体感染的一种极有价值的方法。
Conclusion: It is suggested that PCR in detecting chlamydozoa trachomatis appears to be more sensitive and more rapid than gold-labelled antigen detection method.
分别采用流式细胞计数仪和荧光定量PCR法检测慢性乙型肝炎患者外周血淋巴细胞凋亡率与血清HBVDNA。
The apoptosis of peripheral lymphocytes extracted from chronic hepatitis B patients were analyzed by flow cytometer. Serum HBVDNA was detected by quantitatively PCR.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
快速半定量分析由于分析简便、通用性强而具有广泛的实用价值，本文简介应用能量色散型x荧光光谱法(EDXFS)进行快速半定量分析的几种实用方法。
Rapid semiquantitative analysis has extensive applicable value by simplicity, rapidity and generality. In this paper, we present some means of this research by EDXFS spectrometer.
方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBVDNA、乙肝标志物及alt的变化情况。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
结果荧光定量pcr法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法，其他非肺结核结果阳性率仅为2。
Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non-tuberculosis was only 2.7%.
应用推荐