将麦冬水煎物经乙醇提取后，色谱曲线的背景可显著降低。
After extracting the decoction with ethyl alcohol, the background (base line) of chromatogram was obviously reduced.
结果麦冬水煎液的直接色谱分析会产生较高的色谱曲线背景。
ResultsIn the HPLC analysis, Radix ophiopogonis decoction could lead to comparatively high background of chromatogram.
讨论了二维色谱重叠峰中背景的检测与扣除、纯色谱曲线的分辨和组分的定性定量分析。
The inspection and deduction of background, the resolution of overlapping peaks and the qualitative and quantitative analysis for two dimension chromatography were discussed.
方法：用HPLCDAD联用色谱检测，借助HELP法解析，基于组分的色谱保留时间和光谱曲线进行定性，然后用总体积积分法定量。
METHODS: To carry out qualitative and quantitative analysis by means of HPLC DAD detection, heuristic evolving latent projections (HELP) resolution method and overall volume integration method.
方法：用HPLCDAD联用色谱检测，借助HELP法解析，基于组分的色谱保留时间和光谱曲线进行定性，然后用总体积积分法定量。
METHODS: To carry out qualitative and quantitative analysis by means of HPLC DAD detection, heuristic evolving latent projections (HELP) resolution method and overall volume integration method.
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