采用荧光分光光度计动态测量不同条件下各组心肌细胞内游离钙离子浓度的变化。
The changes of the intracellular concentration of free calcium ion in myocardial cells in each group under different conditions were dynamically measured with spectrofluorometer.
方法：采用荧光显微镜、电子显微镜、激光扫描共聚焦显微镜、流式细胞分光光度计和DNA凝胶电泳等方法和技术观察细胞凋亡。
Method: Using fluorescence microscopy, electron microscopy, laser scanning confocal microscopy, flow cytometry and DNA gel electrophoresis to demonstrate the presence of apoptosis.
评估神经细胞变化的指标检测：采用显微荧光分光光度计测定细胞老化指标脂褐素水平（相对荧光值）反映细胞老化程度；
The evaluation of nerve cell aging index: The level of lipofuscin (relative fluorescent value) index of cells aging was detected by micro-spectrofluorometer to reflect the degree of cell aging;
评估神经细胞变化的指标检测：采用显微荧光分光光度计测定细胞老化指标脂褐素水平（相对荧光值）反映细胞老化程度；
The evaluation of nerve cell aging index: The level of lipofuscin (relative fluorescent value) index of cells aging was detected by micro-spectrofluorometer to reflect the degree of cell aging;
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