使用淋巴细胞分离液和3%的明胶分离胎儿细胞。
Fetal cells were isolated using lymphocyte separation liquid and 3% gelatin.
利用淋巴细胞分离液分离出单核细胞,采用贴壁生长法分离纯化内皮祖细胞。
Lymphocyte separation medium was used to isolate the mononuclear cells, and the attachment growth method was used to purify endothelial progenitor cells.
方法:实验采用干细胞分离液,利用梯度密度法分离患者自体骨髓干细胞,制备干细胞悬液。
METHODS: Autologous BMSCs were isolated by density gradient centrifugation with stem cell extract liquid to prepare stem cell suspension, and were intramuscularly injected into the suffered limbs.
无菌条件下取正常足月剖宫产胎儿的脐带血,经肝素抗凝,用淋巴细胞分离液分离脐血单个核细胞。
The specimens were obtained sterilely with preservative-free heparin, and the cord blood mononuclear cell was isolated by lymphocyte separation medium.
淋巴细胞分离液能够简单,快速的从全血中分离淋巴细胞,并且是经过稀释以及用抗凝剂或除去血液中的纤维蛋白试剂处理的。
Lymphosep is designed for the simple, rapid isolation of lymphocytes from whole blood that has been diluted and treated with anti-coagulant or defibrinating agent.
正像犹他州立大学的研究一样,ReCell方法从活组织切片样本中分离出基础细胞和角化细胞,生产出可喷涂的酶悬浮液,。
Much like the Utah study, the ReCell process dissolves basal cells and keratinocytes from a biopsy sample, producing a sprayable enzyme suspension.
方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
本文就胚胎质量和胚胎类型分化抑制物培养基传代时间和消化液等方面对胚胎干细胞分离克隆的影响进行了讨论。
Influences of embryo quality and type differentiation stayer media passage and digestion fluid on embryonic cell separation and cloning were discussed respectively.
方法用酶消化、分离鸡胚关节软骨细胞,体外单层培养,收集细胞培养液。
Methods Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. Chondrocyte culture media was collected.
应用酶解技术将纤维质和果胶质水解,提取出细胞质和细胞间液,再经分离纯化可提取海带多糖。
The cytoplasm and intercellular fluid can be extracted from enzyme hydrolyzed cellulosic material and pectin, then the laminarin can be obtained via separation and purification.
从细胞萃取液或生物液体中分离酶的方法,基本上就是纯化蛋白质的方法。
The methods employed fOR enzyme isolation from cell extracts OR from biological fluids are essentially those fOR the purification of proteins.
制备复合材料颗粒的悬液,分离和培养兔间充质干细胞。
The suspension of the composite particles was made. The rabbit mesenchymal stem cells were isolated and cultured.
的分离液,并通过无菌采集患卡氏住白细胞虫病的病鸡血液获得了卡氏住白细胞虫的配子体。
In this study, the gametocytes of Leucocytozoon caulleryi were isolated from blood of diseased chickens by density gradient centrifugation.
通过比较LIF和大鼠心肌条件液在小鼠es细胞分离培养过程中的差异,从而选择较为合适的培养液用于小鼠es细胞的分离培养与深入研究。
By comparing the difference between LIF media conditioned and rat cardiomyocyte media conditioned during isolation and culture of mouse ES cell, suitable media is selected in the future.
方法通过全骨髓培养方法分离培养兔骨髓基质细胞,并在培养瓶中进行扩增,使用成骨诱导液作为培养基;
Methods Rabbit marrow stromal cells were isolated through whole marrow culture method and amplified in flasks, using the osteoblast inducing conditional media served as the culture media.
无菌采集正常分娩或剖宫产胎儿脐带血,分离干细胞,经肝动脉介入将干细胞悬液定植入肝脏。
The therapeutic stem cells were collected and isolated from the umbilical blood of fetal delivered by normal or uterine-incision woman.
本文首先用多次联合消化脂肪组织的方法分离培养脂肪干细胞,通过换液的方法去除红细胞,减小了红细胞裂解液对细胞的损伤。
Human ADSCs were isolated firstly in this study with improved method, through the exchange of the cell medium to remove the red blood cells, reducing the damage of cells.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞,免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法:采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞,并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法:采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞,并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
应用推荐