目的:探讨消减免疫法在分析肝癌细胞凋亡反应中瞬时表达的某些相关抗原分子的作用。
Aim: to explore the role of subtractive immunization techniques in analysis of some transiently expressed antigens of hepatoma apoptotic cells during apoptotic response.
随后的分析显示它的分子是一种抑制细胞凋亡,或者细胞程序性死亡。
A subsequent analysis of the molecule revealed it to be an inhibitor of apoptosis, or programmed cell death.
用琼脂糖凝胶电泳法分析凋亡细胞的DNA梯带。
Then DNA ladder zone of apoptosis cells was analyzed with the method of agarose gel electrophoresis.
用MTT法分析顺铂对转染前后的8348细胞抑制增殖和诱导凋亡的能力。
Proliferation suppression and apoptosis of 8348 cells pre and post transfection induced by cisplatin were analysed by MTT methods.
用自动图像分析系统,对25例大肠肿瘤的细胞凋亡和形态计量学参数进行了研究。
The cellular apoptosis and morphometry of 25 cases of colorectal neoplasms were studied with automatic image analyser.
结果经图像分析,药物防治组的肝纤维化程度显著降低,细胞凋亡数量显著减少(均P<0.01)。
Results By an image analysis system, the number of hepatocyte apoptosis of the treatment group was more less than that of the control group(P<0.01).
流式细胞仪进行凋亡细胞百分数分析。
The percent of the apoptosis cell was analyzed by Flowcytometry.
用流式细胞仪分析细胞周期与凋亡状况。
本研究探讨苦参碱诱导T淋巴细胞白血病JM细胞凋亡相关基因的表达变化,并进一步分析其机制。
This study was purposed to investigate the changes of apoptosis-related gene expression in t lymphocytic leukemia JM cells induced with matrine, and its possible mechanism.
结果形态学变化、流式细胞仪分析和TUNEL法结果均证实含鲜壁虎血清在体外可诱导C6胶质瘤细胞凋亡;
Results The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method.
目的通过检测儿童急性白血病多药耐药基因及细胞凋亡调控基因表达水平的改变,分析不同类型白血病的主要耐药机制。
Objective To study the drug resistance in different types of acute leukemia in children, the expression of multidrug resistance gene and apoptosis-related genes were analyzed.
目的比较分析游走性舌炎病损不同部位的表层脱落细胞在分化、增殖、凋亡上的差异。
Objective (1) to analyze the differences of differentiation, proliferation and apoptosis in exfoliated cells from different areas of migratory glossitis.
结果①流式细胞术分选后共聚焦显微镜分析技术(PSC)能同时、准确地鉴别不同细胞周期时相的凋亡细胞和非凋亡细胞。
Results Post-sorting confocal (PSC) method could exactly distinguish apoptotic and non-apoptotic cells in the specific cell cycle phases at the same time.
流式细胞仪分析细胞凋亡。
方法采用核固红结晶紫染色方法,原位末端标记法及形态计量方法定量分析了被动吸烟致金黄地鼠神经管上皮细胞凋亡的变化。
Methods By using the nuclear fast red-crystal violet staining method and situ TUNEL technology, the quantitative changes of apoptosis in neural epithelium in gold hamster were analyzed.
目的:研究耐力训练对大鼠力竭运动后淋巴细胞凋亡的影响,并分析其机制。
Objective Effects of endurance swimming training on the exhaustion-induced lymphocyte apoptosis were investigated in rats and the mechanism was also analyzed.
以流式细胞仪和荧光显微镜分析细胞凋亡状况。
Apoptosis was analyzed with FACS and fluorescent microscope.
应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。
The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method.
细胞周期分析图中出现凋亡峰;
The subdiploid peaks due to apoptosis were also found in cell cycle analyses.
我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证,证实了基因芯片结果的可靠性。
We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.
用流式细胞仪分析并测定细胞凋亡率;
细胞周期分析和DNA电泳研究细胞凋亡。
The apoptosis of NB4 cell was analyzed by DNA fragments and FACS.
分析CD4+CD28-T细胞比例与外周血淋巴细胞凋亡率的相关性。
The correlation between the fraction of CD4+CD28-T cells and lymphocytic apoptosis was also observed.
用流式细胞术分析奥曲肽对胃癌细胞周期分布及凋亡率的影响;
The influence of octreotide on cell cycle distribution and the apoptosis rate of human gastric carcinoma cell were analyzed with FCM.
应用TUNEL技术检测其中的43例NSCLC癌细胞的凋亡并分析其与CC10表达的相关性。
The 43 NSCLC apoptosis were detected by TUNELtechnique. analysing correlation between the 43 NSCLC apoptosis index (AI) and CC10 protein expression.
研究表明PEA - 15表达抑制了细胞增殖,细胞循环分析没有揭示凋亡而揭示了自体吞噬,这是通过LC3的切割确认的。
PEA-15 expression inhibited proliferation, and cell cycle analysis did not reveal apoptosis but did reveal autophagy, which was confirmed by an increase in LC3 cleavage.
体外实验应用MTT法、观察细胞形态、测定细胞生长曲线、流式细胞仪分析细胞凋亡及细胞周期观察提取物6320对不同肿瘤细胞生长的影响。
In vitro, MTT assay, cell morphological changes, cell growth curve were used to observe the inhibitory effects of Extract 6320 on tumor cells.
流式细胞术(FCM)分析细胞周期及细胞凋亡的变化。
The changes of cell cycle and apoptosis were analyzed by flow cytometry (FCM).
应用流式细胞术进行DNA倍体分析和TUNEL分析,检测两药单用与联用对细胞周期及早期凋亡的影响;
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
FCM分析发现细胞在感染腺病毒后出现 细胞周期阻滞和细胞凋亡。
The cell cycle arrest and the cell apoptosis were found by FCM.
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