通过单因素试验确定紫外线诱变的时间，之后对T-3纳豆杆菌进行诱变处理。
Through the single factor experiment determination ultraviolet ray mutagenesis's time, and under this mutagenesis time's condition, the natto bacillus carries on mutafacient processing to T-3.
纳豆激酶是纳豆杆菌在发酵过程中产生的一种丝氨酸蛋白酶，具有良好的纤溶酶活性。
The natto activating enzyme was the serine proteinase which produces in the natto bacillus fermentative process, has the good filament solubility.
方法从纳豆芽胞杆菌中分离纯化基因组DNA ，作为模板通过PCR扩增纳豆激酶原基因。
Methods Chromosome DNA was isolated from Bacillus natto . The pro nattokinase gene was then amplified from chromosome by PCR.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的：利用基因工程技术，构建表达具溶栓活性的纳豆激酶的大肠杆菌工程菌。
AIM: to construct engineered E. coli strains which can express nattokinase with fibrinolysis activity using gene engineering technology.
目的：利用基因工程技术，构建表达具溶栓活性的纳豆激酶的大肠杆菌工程菌。
AIM: to construct engineered E. coli strains which can express nattokinase with fibrinolysis activity using gene engineering technology.
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