表达的融合蛋白约占菌体蛋白总量的2 8%。
以纯化的融合蛋白为抗原免疫家兔制备出了抗血清。
结果:经表达和纯化获得了纯度达90%以上的融合蛋白;
Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.
该单克隆抗体是通过用包含人EGF受体胞质域的融合蛋白免疫动物而制备的。
Monoclonal antibody is produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human EGF receptor.
本发明的融合蛋白在用于检测时敏感性好且无需避光操作,不存在放射等污染因素。
The invention has good sensitivity during detecting stage without doing photopathic operation, which doesn't display radioactive pollution.
因此我们制备了ICA69的融合蛋白,可以直接促进ICA69和PICK1的结合。
Thus, we prepared fusion protein of ICA69 that would directly enhance the combining between ICA69 and PICK1.
在诱导表达优化条件下,该重组菌可产生表达量占细菌总蛋白量30%以上的融合蛋白。
Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;
薄层凝胶光密度扫描分析结果显示 ,表达的融合蛋白量约占细菌总蛋白的 2 6%。
The portion of the fusion protein accounted for 26% of all the proteins by thin layer gel optical scanning.
结论:构建了8r - MUC1核心肽融合蛋白原核表达载体并成功表达与纯化出具有生物学活性的融合蛋白。
Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.
将表达的融合蛋白作为包被抗原,建立了间接酶联免疫吸附试验(间接elisa)的诊断方法以检测CDV血清抗体水平。
The method of indirect ELISA to detect CDV antibody in blood serum was established, using recombinant nucleocapsid protein as envelope antigen.
结论:利用杆状病毒表达系统,成功地表达同时具有核蛋白及糖蛋白g 2生物学活性的融合蛋白g2s0 . 7,为进一步研究其免疫学特性奠定了基础。
CONCLUSION: the successful expression of fusion protein G2S0.7 with biological activity in insect cells, which lays the foundation for further research on its immunological characteristic.
对重组乙型肝炎(乙肝) 病毒前S1抗原(1- 42 )及核心抗原(1- 144 )表达的融合蛋白CS1进行了免疫原性的研究分析,以便为探索HBV治疗性疫苗的研制提供实验依据。
The aim of this study was to express a fusion protein of HBV preS1 Ag(1-42) and HBcAg(1-144) and analyze its immunogenicity for further study of HBV therapeutic vaccine.
现在对这种病已有针对性疗法,Gleevec大体是过去十年中效果最显著的药物,它能使另一融合基因BCR-ABL的编码蛋白失去活性。
There is already a targeted treatment for this disease. Gleevec, probably the most remarkable anti-cancer drug of the past decade, disables the protein encoded by another fused gene, BCR-ABL.
以EDDIE为融合蛋白是在大肠杆菌中高效表达抗菌肽的一种好方法。
This method provides an excellent way for high expression of antimicrobial peptides when fused with EDDIE.
研究人员已经研制出一种比绿色荧光蛋白小的蓝色荧光探针,它更容易标记活细胞中肽融合重组蛋白。
Researchers have engineered a blue fluorescent probe that is smaller than GFP and will improve the labeling of peptide-fused recombinant proteins in living cells.
由于LAP2肽可以融合在一定数量的不同蛋白上,该方法能够选择性标记在某些特定的亚细胞亚群的蛋白。
Because the LAP2 peptide can be fused to a number of different proteins, the method enables the selective labeling of certain subpopulations of proteins in specific subcellular compartments.
本文论述了基因重组融合蛋白纯化过程和存在的问题。
The paper describes process of recombinant fusion protein in purification and existing problems.
结论GRA7基因在大肠埃希菌中以gst融合蛋白的形式得到表达。
Conclusion The GRA7 gene may be expressed as a GST fusion protein in E. coli.
结果表明,用此融合蛋白的包涵体免疫豚鼠能诱导产生中和抗体,并对病毒的攻击提供免疫保护。
The results showed that the fusion protein can yield antibodies with high neutralizing activity as well as provide protection against challenge with virulent virus.
已明确脂滴在细胞内融合过程中包含了一种称作“SNAP23”的蛋白。
It became clear that the lipid droplets merged with each other inside the cell by a process that involved a protein known as "SNAP23".
目的:表达人免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白,制备高质量的兔抗人IRGM多克隆抗体。
Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.
结果:成功克隆了CAT的基因片段,并在大肠杆菌中得到其融合蛋白的表达。
Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.
一种在研治疗湿性amd的新药是一种重组融合蛋白,它能结合眼内血管形成关键生长因子蛋白。
A new medicine in development for the wet form of AMD is a recombinant fusion protein that binds to the growth factor protein that plays a critical role in blood-vessel formation in the eye.
通过融合两个或多个不同蛋白产生的蛋白质。
A protein that is made by fusing together two or more different proteins.
MTT法检测融合蛋白对黑色素瘤细胞的体外抑制率。
The inhibition ratio of the fusion protein was tested by MTT method.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
建立了TSF蛋白的纯化复性方法,获得了GST - TSF融合蛋白及TSF蛋白。
The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.
建立了TSF蛋白的纯化复性方法,获得了GST - TSF融合蛋白及TSF蛋白。
The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.
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