• 表达融合蛋白约占菌体蛋白总量2 8%。

    The yield of the fused protein was 28% of total proteins.

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  • 纯化的融合蛋白抗原免疫家兔制备出了抗血清。

    The purified protein was used as antigen to immune rabbits.

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  • 结果表达纯化获得纯度达90%以上融合蛋白

    Results: The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography.

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  • 该单克隆抗体通过包含EGF受体胞质融合蛋白免疫动物而制备

    Monoclonal antibody is produced by immunizing animals with a fusion protein containing the cytoplasmic domain of human EGF receptor.

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  • 发明的融合蛋白用于检测敏感性无需操作存在放射污染因素。

    The invention has good sensitivity during detecting stage without doing photopathic operation, which doesn't display radioactive pollution.

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  • 因此我们制备了ICA69融合蛋白可以直接促进ICA69PICK1结合

    Thus, we prepared fusion protein of ICA69 that would directly enhance the combining between ICA69 and PICK1.

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  • 诱导表达优化条件下重组可产生表达细菌蛋白量30%以上融合蛋白

    Under the optimized expression condition, the recombinant expressed product is account for over 30% of overall bacterium protein;

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  • 凝胶密度扫描分析结果显示 ,表达融合蛋白量约细菌总蛋白 2 6%。

    The portion of the fusion protein accounted for 26% of all the proteins by thin layer gel optical scanning.

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  • 结论:构建8r - MUC1核心融合蛋白表达载体成功表达纯化出具有生物学活性融合蛋白

    Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.

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  • 表达融合蛋白作为抗原,建立间接酶联免疫吸附试验(间接elisa)诊断方法检测CDV血清抗体水平

    The method of indirect ELISA to detect CDV antibody in blood serum was established, using recombinant nucleocapsid protein as envelope antigen.

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  • 结论:利用杆状病毒表达系统,成功地表达同时具有蛋白及糖蛋白g 2生物学活性融合蛋白g2s0 . 7,进一步研究免疫学特性奠定基础

    CONCLUSION: the successful expression of fusion protein G2S0.7 with biological activity in insect cells, which lays the foundation for further research on its immunological characteristic.

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  • 重组乙型肝炎(乙肝) 病毒前S1抗原1- 42 )核心抗原(1- 144 )表达融合蛋白CS1进行了免疫原性研究分析,以便为探索HBV治疗疫苗研制提供实验依据。

    The aim of this study was to express a fusion protein of HBV preS1 Ag(1-42) and HBcAg(1-144) and analyze its immunogenicity for further study of HBV therapeutic vaccine.

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  • 现在这种已有针对性疗法Gleevec大体过去十年中效果显著药物,它能使另一融合基因BCR-ABL编码蛋白失去活性。

    There is already a targeted treatment for this disease. Gleevec, probably the most remarkable anti-cancer drug of the past decade, disables the protein encoded by another fused gene, BCR-ABL.

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  • EDDIE为融合蛋白大肠杆菌高效表达抗菌方法

    This method provides an excellent way for high expression of antimicrobial peptides when fused with EDDIE.

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  • 研究人员已经研制出一种绿色荧光蛋白蓝色荧光探针,它更容易标记细胞融合重组蛋白

    Researchers have engineered a blue fluorescent probe that is smaller than GFP and will improve the labeling of peptide-fused recombinant proteins in living cells.

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  • 由于LAP2可以融合在一定数量不同蛋白上,方法能够选择性标记在某些特定亚细胞亚蛋白

    Because the LAP2 peptide can be fused to a number of different proteins, the method enables the selective labeling of certain subpopulations of proteins in specific subcellular compartments.

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  • 本文论述了基因重组融合蛋白纯化过程存在问题

    The paper describes process of recombinant fusion protein in purification and existing problems.

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  • 结论GRA7基因大肠埃希菌中以gst融合蛋白形式得到表达

    Conclusion The GRA7 gene may be expressed as a GST fusion protein in E. coli.

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  • 结果表明融合蛋白包涵体免疫豚鼠诱导产生中和抗体,并病毒攻击提供免疫保护

    The results showed that the fusion protein can yield antibodies with high neutralizing activity as well as provide protection against challenge with virulent virus.

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  • 明确细胞融合过程包含称作SNAP23蛋白

    It became clear that the lipid droplets merged with each other inside the cell by a process that involved a protein known as "SNAP23".

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  • 表达免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白制备质量抗人IRGM多克隆抗体

    Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.

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  • 结果:成功克隆CAT基因片段,并大肠杆菌中得到融合蛋白表达

    Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.

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  • 种在研治疗湿amd新药一种重组融合蛋白能结合血管形成关键生长因子蛋白

    A new medicine in development for the wet form of AMD is a recombinant fusion protein that binds to the growth factor protein that plays a critical role in blood-vessel formation in the eye.

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  • 通过融合两个多个不同蛋白产生蛋白

    A protein that is made by fusing together two or more different proteins.

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  • MTT检测融合蛋白黑色素瘤细胞体外抑制

    The inhibition ratio of the fusion protein was tested by MTT method.

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  • 胰岛素基因融合到金色葡萄球菌蛋白a基因上,构建大肠杆菌中基因融合外分泌表达载体

    The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.

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  • 建立了TSF蛋白纯化复性方法获得GST - TSF融合蛋白及TSF蛋白

    The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.

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  • 建立了TSF蛋白纯化复性方法获得GST - TSF融合蛋白及TSF蛋白

    The purification procedure was established, and the purified TSF and its fused protein GST TSF were obtained.

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