方法：采集健康的牙龈组织和牙周膜，体外培养牙龈成纤维细胞和牙周膜成纤维细胞。
Methods: Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro.
目的：观察模拟咬合压力对人牙周膜成纤维细胞环氧化酶2 (COX 2)基因表达的影响。
Objective: To investigate the influence of simulated occlusion pressure on the cyclooxygenase-2 (COX-2) gene expression in human periodontal ligament fibroblasts (HPLFs).
牙再植术的历史悠久，但是脱位牙根面部分牙周膜细胞活性丧失、牙根吸收，影响着牙再植术的成功率。
But the lose of the activity of periodontal ligament cells and root replantation has influenced the successful rate of tooth replantation for a long time.
目的：观察中间普氏菌和脆弱类杆菌的内毒素对人牙周膜细胞增殖和超微结构的影响。
AIM: to examine the effect of endotoxin of prevotella intermedia and bacteroides fragilis on proliferation and ultrastructure of periodontal ligament cells in vitro.
结论：SP - PLGA纳米微球通过持续释放活性SP，可在较长时间内促进牙周膜成纤维细胞的增殖。
Conclusion: It suggested SP-PLGA can promote the proliferation of human periodontal ligament fibroblasts through a long period of controlled release of SP.
通过考马斯亮蓝法测定牙周膜细胞总蛋白含量，透射电子显微镜观察细胞超微结构的变化。
Commasie brilliant blue staining was used to study the total protein content and transmission electron microscopy was used to study the ultrastructure of PDLCs.
目的：建立免疫磁珠法体外分离纯化人牙周膜干细胞的方法。
Objective: To isolate and identify the periodontal ligament stem cells from human molars.
它还可诱导牙周膜细胞的增殖和分化。
Furthermore, it can also stimulate proliferation and differentiation of periodontal ligament cell.
考马斯亮蓝法测定人牙周膜细胞的总蛋白含量。
Then the total protein content was measured by using Coomassie brilliant blue staining.
结论：由于增龄的影响，老年人牙周膜细胞生长增殖功能明显降低。
Conclusions: Under the influence of ageing, proliferation ability of elderly peoples periodontal ligament cells was significantly decreased and was significantly different from that of young peoples.
本研究中，我们尝试利用自体牙周膜干细胞治疗小猪牙周病模型上的牙周缺损。
In this study, we explored the potential of using autologous periodontal ligament stem cells (PDLSCs) to treat periodontal defects in a porcine model of periodontitis.
拔除小型猪的牙齿取自体的牙周膜干细胞，体外培养扩增。
Autologous PDLSCs were obtained from extracted teeth of the miniature pigs and then expanded ex vivo to enrich PDLSC Numbers.
牙周组织再生重建的关键是牙周膜细胞的增殖分化。
The key of periodontal tissue regeneration is the proliferation and differentiation of human periodontal ligament fibroblasts (HPDLFs) .
牙周组织再生重建的关键是牙周膜细胞的增殖分化。
The key of periodontal tissue regeneration is the proliferation and differentiation of human periodontal ligament fibroblasts (HPDLFs) .
应用推荐