方法提取肿瘤组织总rna，用反转录聚合酶链式反应(RT - pcr)法进行检测。
Methods Total RNAs of tumor tissues were extracted and were detected by RT-PCR.
干预：40只大鼠，于伤后不同时间取水肿区脑组织及正常脑组织：提取组织总rna：用共扩增反转录定量pcr方法测定MMP - 9基因表达水平。
INTERVENTIONS: Tissues of edema area and normal brain of 40 rats were collected in different time after damage: total RNA were abstracted and MMP-9 gene expression was tested by PCR means.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
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