• 生物结合位点复杂性结构不规则使得最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性(4)。

    The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

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  • 方法直接包被SPA法、生物素-亲和法。

    Method: Direct method, SPA method, Biotin Avidin method.

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  • 目的探讨TM5.28单克隆抗体-生物素-亲和系统检测日本血吸虫循环抗原及其诊断效果

    Objective To explore the diagnostic efficiency of circulating antigen using the TM5. 28 mAB-biotin-avidin system for the detection of schistosomiasis japonica.

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  • 因为的强亲和力非共价结合链霉素亲和可以用于检测和分离生物素蛋白(1.2)。

    Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).

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  • 蛋白质亲和结合生物素亲和部分无关

    The binding of the protein to avidin is not related to biotin or the oligosaccharide chain of avidin.

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  • 利用生物素-亲和生物放大系统的原理,建立新的检测细胞培养的甲型肝炎病毒抗原的方法

    A new technique used to detect HAAg in human diploid embryonic lung fibroblast ( HDLF ) cell culture was developed.

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  • 链霉亲和是从链霉生物蛋白分离出来53,000道尔顿的同源四聚体(3),一个链霉亲和的亚基可以维生形成高亲和力非共价结合。

    Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity.

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  • 在用于实际试样的回收率测定,结果良好。(2)提出一种基于催化沉积底物生物素-亲和两步放大灵敏免疫检测方法(第3)。

    An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).

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  • 通过对比不同化学固定方式,选择亲和素-生物素作为适配子探针固定方法。

    Bycomparing different chemical methods for the immobilization of aptamer, biotin-avidin method was finally chosen.

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  • 采用链霉亲和纳米粒子,将生物素标记特异性抗体联在磁纳米粒子上,制备出高特异性纳米探针

    The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.

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  • 采用链霉亲和纳米粒子,将生物素标记特异性抗体联在磁纳米粒子上,制备出高特异性纳米探针

    The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.

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