生物素结合位点的复杂性和结构的不规则性使得它最适合与生物素结合并且表现出与链霉素亲和素-生物素复合体的高度特异性(4)。
The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).
方法:直接包被法、SPA法、生物素-亲和素法。
目的探讨TM5.28单克隆抗体-生物素-亲和素系统检测日本血吸虫循环抗原及其诊断效果。
Objective To explore the diagnostic efficiency of circulating antigen using the TM5. 28 mAB-biotin-avidin system for the detection of schistosomiasis japonica.
因为它的强高亲和力非共价结合,链霉素亲和素可以用于检测和分离生物素化的蛋白(1.2)。
Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).
该蛋白质与亲和素的结合与生物素及亲和素的糖链部分无关。
The binding of the protein to avidin is not related to biotin or the oligosaccharide chain of avidin.
利用生物素-亲和素生物放大系统的原理,建立了新的检测细胞培养的甲型肝炎病毒抗原的方法。
A new technique used to detect HAAg in human diploid embryonic lung fibroblast ( HDLF ) cell culture was developed.
链霉素亲和素是从链霉亲生物素蛋白中分离出来的53,000道尔顿的同源四聚体(3),每一个链霉素亲和素的亚基可以与维生素形成高亲和力非共价结合。
Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity.
在用于实际试样的回收率测定中,结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
通过对比不同化学固定方式,选择亲和素-生物素法作为适配子探针的固定方法。
Bycomparing different chemical methods for the immobilization of aptamer, biotin-avidin method was finally chosen.
采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
采用链霉亲和素包被磁纳米粒子,将生物素标记的特异性抗体偶联在磁纳米粒子上,制备出高特异性的磁纳米探针;
The magnetic nanoparticle probe was prepared by specifically connecting the streptavidin-conjugated magnetic nanoparticles and the antibody of analyte via the strong streptavidin-biotin interaction.
应用推荐