研究中使用的测序方法允许科学家们同时测定上百万组基因序列。
The sequencing process used allows scientists to create up to millions of sequences at the same time.
2002年人们完成了五年前开始的脊髓灰质炎病毒的基因组全序列的测定,三年后同样规模的一个团队只花了一周的时间做了一个相似长度的基因组测序。
In 2002 it took five years to develop the genomic sequence of a polio virus. Three years later it took a week for a team the same size to do the same on a virus of similar length.
方法利用聚合酶链反应(PCR)产物直接测序,对10例血液标本进行了HLA-DPB1、DRB外显子2的序列分析,并将测定结果与基因型核酸序列数据库进行比较。
Methods Sequences of HLA-DPB1, DRB exon2 of 10 samples were analysed by direct sequencing of PCR products. The results of sequencing were compared with their corresponding sequence data.
方法设计出针对各片段的特异性引物,用P CR方法从Z 37病毒感染的细胞提取细胞总rna,逆转录扩增、产物克隆t载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
选择5份标本进行VP1 - 2a基因片段序列测定,测序结果经过比对后发现,5株的VP1 - 2 A序列完全一致。
VP1-2A nucleotide fragments from 5 samples were cloned and sequenced. The results showed that VP1-2A nucleotide fragments of 5 strains were identical.
选择5份标本进行VP1 - 2a基因片段序列测定,测序结果经过比对后发现,5株的VP1 - 2 A序列完全一致。
VP1-2A nucleotide fragments from 5 samples were cloned and sequenced. The results showed that VP1-2A nucleotide fragments of 5 strains were identical.
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