本文实现了重组别藻蓝蛋白基因在毕赤酵母中的异源表达。
In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.
毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
将正确连接的表达质粒线性化后用电穿孔法转化到毕赤酵母GS115中。
The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation.
结果剔除毕赤酵母中的PEP4基因后外源蛋白质表达量平均提高9．5%。
Results By disrupting the PEP4 gene in Pichia pastoris, the productivity of heterologous protein expression increased 9.5% on average.
目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
结论毕赤酵母系统重组表达的膜联蛋白V具有较高的结合外露PS红细胞的能力。
Conclusion The annexin V expressed recombinantly in pichia pastoris show higher affinity to PS exposed erythrocytes.
将其展示于毕赤酵母表面展示，不但实现了自固定化，耐热性和催化活性也有改善。
Displayed on the pichia surface, CALB not only achieved self-immobilization, also has it's heat resistance and catalytic activity improved.
纯化的重组菊粉酶的其他生物化学性质与海洋季也蒙毕赤酵母中的野生型菊粉酶相一致。
Other biochemical characteristics of the purified recombinant inulinase were the same as those of the native inulinase produced by the marine derived P. guilliermondii strain 1.
结论：为下一步在毕赤酵母中组成型表达外源蛋白，研究其作用机理和遗传机制奠定了基础。
Conclusion:The GAP promoter can be used to express foreign protein effectively and is the basis for studying the physicochemical properties and genetic mechanism of Gassericin T.
结果表明，与对葡糖糖的利用相比，树干毕赤酵母菌利用内醚糖的效率较低，且不能发酵产乙醇。
Results revealed that P. stipitis could utilize levoglucosan at a low efficiency compared to glucose, and could not ferment it to ethanol.
其与酿酒酵母gpd 1基因同源性高达60%，与安格斯毕赤酵母gpd基因同源性高达70%。
The gene has homology as high as 60 percent with saccharomyces cerevisia GPD1 gene and 70 percent with Angus pichia yeast GPD gene.
同时，在毕赤酵母里表达抗菌肽的研究为开发转基因酵母作为鱼类饵料添加剂的应用研究提供了理论基础。
Moreover, recombinant expression of antimicrobial peptides in yeast provides us an opportunity to explore the recombinant strains as additive feedstuff of fishery.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
目的：利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白，为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
目的：利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白，为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
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