除了低克隆率-只有1.7%的胚胎能存活到最后阶段-创造转基因狗还有一个挑战即如何控制外部基因在核d NA中的植入位置。
Besides the low efficiency of cloning - just 1.7 per cent of embryos came to term - another challenge to creating transgenic dogs is controlling where in the nuclear DNA a foreign gene lands.
由于缺乏一个保存完整的细胞核，这使得对她进行像多利羊那样的克隆是不可能了。
The lack of an intact nucleus makes cloning a la Dolly the sheep impossible.
科学家们从死鼠的器官中提取细胞核，移入到另一只活鼠的卵细胞中，最终克隆出了小鼠。
The scientists extracted a cell nucleus from an organ of a dead mouse and planted it into the egg of another mouse which was alive, leading to the birth of the cloned mouse.
NBAC的成员明确表示，他们呼吁由私人提供资金的研究人员和机构不要试图通过人体细胞核转移去克隆人。
NBAC members also indicated that they would appeal to privately funded researchers and clinics not to try to clone humans by body cell nuclear transfer.
科学家们就通过人体细胞核转移去克隆人的问题争论不休。
Scientists have been warring over the issue of cloning a human child by body cell nuclear transfer.
目的克隆与原核表达“牵引丝蛋白基因”单体六聚物，为开展具有不同长度系列的“牵引丝蛋白基因”单体多聚物的功能研究奠定基础。
Objective To clone and express the hexamer of the gene of spider dragline silk protein, as a foundation of further research on the functions of the dragline silk protein gene of different lengths.
随着核转移技术的提高，克隆能大规模改善农业工业。
Cloning could massively improve the agricultural industry as the technique of nuclear transfer improves.
克隆羊“多莉”的问世是应用了一项称为细胞核转移的技术。
Dolly the sheep was cloned using a technique called nuclear transfer.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
结论原核表达系统可有效克隆较高纯度的CHM - I基因。
Conclusion The more effective CHM-I clone with high purity could be expressed by procaryotic expression system.
核转移也能为需要器官移植的儿童提供克隆产生的捐赠器。
Nuclear transfer could also provide children who need organ transplants to have a clone born to donate organs.
目的：建立肾综合征出血热(HFRS)患者汉滩病毒(HTNV)核衣壳蛋白(NP)特异性CTL克隆的靶细胞。
AIM: To establish the target cells of CTL clone which is specific for the nucleocapsid protein (NP) of Hantaan virus (HTNV) in patients with hemorrhagic fever with renal syndrome (HFRS).
EIN3基因首先是从拟南芥的突变体中克隆得到的，作用于CTR下游，位于核内，编码转录因子。
EIN3 was first cloned in Arabidopsis thalina mutant, and it has been suggested that EIN3 acted downstream to CTR located in nuclear and encoded transcription factors.
结论：成功克隆SARS冠状病毒核衣壳蛋白基因全长，并在酿酒酵母中诱导表达成功，有助于抗sars分子疫苗的研究。
Conclusion Successful clone of an integrity SARS-CoV nucleocapsid gene and its effective expression in saccharomyces cerevisiae can be beneficial to the research on SARS vaccine.
自1985年克隆出了第一个冰核基因后，目前已经从冰核细菌中克隆了8个冰蛋白基因并完成了测序。
First active gene had been cloned by 1985. After that, the 8 ice genes had been gotten and sequenced in the past years.
目的克隆人热休克蛋白72 (HSP72)基因，原核表达并纯化蛋白产物，以探讨其生物学功能。
AIM To clone human heat shock protein 72 (HSP72) gene, do prokaryotic expression and purify HSP72 protein for further study.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的：克隆杜氏盐藻核基质附着区(MAR)结合蛋白片段。
Aim: To clone MAR-binding protein cDNA fragment from Dunaliella salina.
目的克隆srg基因、原核表达并鉴定。
目的从人外周血淋巴细胞中克隆出cd 81基因，构建真核表达质粒，并在COS - 7细胞中进行表达。
Aim to clone human CD81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in COS-7 cells.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
通过基因克隆等方法，选择原核表达系统，并对目的蛋白进行分离纯化，初步获得有活性的人内毒素结合肽ebp。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。
Aim to analyze THANK gene expression in peripheral blood mononuclear cells (PBMC) stimulated with different stimulators and to clone whole-length human THANK gene.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
①目的构建重组蜂毒肽的原核表达克隆。
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
因此本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。
The paper mainly reviewed several factors on influencing somatic cloning of pigs, which involve in cytoplast recipients, donor cells, micromanipulation, activation and reconstituted embryo transfer.
因此本文主要综述了影响体细胞克隆猪的几个因素，涉及胞质受体、供核细胞、显微操作、激活以及重组胚移植。
The paper mainly reviewed several factors on influencing somatic cloning of pigs, which involve in cytoplast recipients, donor cells, micromanipulation, activation and reconstituted embryo transfer.
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