为了解人类LDL受体基因内含子15的遗传背景，利用长链PCR和锚定PCR分离了LDL受体基因外显子15-内含子15-外显子16和内含于15的3’末端片段。
To understand intron 15 of human LDL receptor gene, the DNA fragments from exon 15 to exon 16 and the 3' end of intron 15 were amplified with long chain PCR and anchored PCR.
在每一个片段的末端，研究者连上一个荧光蛋白，在另一端他们工程DNA能熄灭荧光的亮度。
At one end of each snippet, the researchers attached a fluorescent protein, and, at the other end, they engineered the DNA so that it could "quench" the fluorescent light.
为了更好地处理低质量片段，BOAT支持一个“3'末端修剪模式”以构建测序片段局部优化的比对，进一步提高灵敏度。
For better handling of low-quality reads, BOAT supports a "3'-end Trimming Mode" to build local optimized alignment for sequencing reads, further improving sensitivity.
用双脱氧链末端终止法测序结果表明，NS 1基因扩增片段5端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。
Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.
生命早期接受母乳喂养的婴儿在4、5岁时端粒(给细胞中染色体末端‘带帽’，用以保护DNA的片段)可能更长。
Infants who are exclusively breastfed early in life are more likely by age 4 or 5 to have longer telomeres, the protective bits of DNA that cap the ends of chromosomes in cells.
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
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