差减阳性克隆的斑点杂交。
Differential DNA dot hybridization of subtractive cDNA clones.
经同源重组后,以终点稀释和斑点杂交法筛选重组杆状病毒;
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
方法:以酶免疫试验和斑点杂交试验检测慢性乙型肝炎(CHB)患者血清hbvm。
Methods: The serum HBVM of patients with CHB were detected with enzyme immunoassay and dot blot hybridization.
膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.49%,特异度为100%。
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%.
同时RNA斑点杂交结果提示在非癌胃组织中随着肠化程度增高人端粒酶rna表达也增强。
At the same time, RNA dot blot revealed that the expression of hTR was increased with intensified intestinal metaplasia in gastric mucosa.
用改良技术研究热适应大鼠下丘脑基因的差异表达,发现了一个差异表达基因片段,斑点杂交证实为阳性片段。
A differentially expressed gene was identified from hypothalamus of heat acclimated rats using the improved technique, which is verified by dot blot.
单株检测,RT - P CR分析,斑点杂交鉴定,确定此片段为花椰菜细胞质雄性不育系knxd612所特有。
Identified by individual plant testing, analysis of RT-PCR and dot blot, this fragment was only existed in CMS cauliflower knxd612.
单株检测,RT - P CR分析,斑点杂交鉴定,确定此片段为花椰菜细胞质雄性不育系knxd612所特有。
Identified by individual plant testing, analysis of RT-PCR and dot blot, this fragment was only existed in CMS cauliflower knxd612.
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