国际首屈一指的BAC文库构建和BAC相关资源服务平台。
An international leading BAC library construction and BAC related resources service platform.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：建立一种改进的更简便、易操作的细菌人工染色体(BAC)文库构建方法。
Objective: To set up a kind of improved method of construction of bacterial artificial chromosome (bac) library.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
本研究在参考了前人BIBAC、TAC和BAC文库构建方法的基础上，通过改进建立了一套可转化大片段文库构建技术体系。
This research has referred to the predecessor in BIBAC, TAC and in the BAC method, established an own set to be possible to transform the big fragment library to construct the technical system.
本研究在参考了前人TAC、BIBAC和BAC文库构建方法的基础上，通过改进建立了一套可转化大片段文库构建技术体系。
This research has referred to the predecessor in TAC, BIBAC and in the BAC method, established an own set to be possible to transform the big fragment library to construct the technical system.
利用这座桥，他可以从文库中挑出一段已知基因，来构建镜像蛋白工具。
With it, he'd be able to pick a known gene from a library and build mirror protein tools.
利用这座桥，他可以从文库中挑出一段已知基因，来构建镜像蛋白工具。
With it, he’d be able to pick a known gene from a library and build mirror protein tools.
他还构建了一个拥有正确结合构象的突变核糖体文库。
And he has a library of mutant ribosomes that have the right kind of sockets-they'll accept mirror amino acids.
构建BAC文库的一个关键步骤就是BAC载体DNA的制备。
A crucial step in BAC library construction is preparation of BAC vector DNA.
结果建立了从骨骼和关节构建RACE c DNA文库的方法，并用该方法成功地构建了人胎儿骨骼和关节RACE c DNA文库。
Results A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed.
该方法为进一步构建中棉所12号多倍基因组的BAC文库、从而进行棉花基因组有关研究奠定了基础。
The method will lay a foundation for more-genome-equivalent BAC library construction of CCRI 12 and cotton genome research.
鸟枪法构建VP3噬菌体全基因组随机文库；
A random library of VP3 DNA was constructed by shot-gun library method.
方法采用RD PCR技术构建SHSY5Y细胞基因片段文库，并对每一个克隆进行测序。
Methods The gene fragment library of SH SY5Y cells was constructed with RD PCR technique.
着重论述了宏基因组学的研究方法，包括DNA的提取、文库的构建以及筛选策略的选择。
Methods in metagenomic study, including DNA extraction, library construction and screening were discussed in this paper.
结论：所构建的基因组d NA消减文库为进一步筛选UPEC132中与致病相关基因奠定了基础。
Conclusion: The DNA subtractive library may provide a solid foundation for screening and cloning pathogenic genes of UPEC132.
目的建立快速有效构建人类染色体区带特异性探针池及其文库的技术。
Objective To establish a rapid and efficient technique of constructing human chromosomal band specific probe pools and their libraries.
对土壤宏基因组DNA的提取、宏基因组文库的构建和筛选等方面的研究现状和进展进行了简要综述。
Progress on the research of metagenomic DNA purification, metagenomic library construction and screening of soil microorganisms are reviewed briefly.
进一步表明，上述的三个条件完全符合构建棉花线粒体基因组细菌人工染色体文库的要求。
Further indicated that, the above three conditions completely conform to the construction cotton mitochondrial DNA bacterial artificial chromosome library requirement.
构建基因组文库是一项非常基础和重要的工作。
目的构建人噬菌体抗体组合文库，筛选人单克隆抗体。
Objective To construct human phage antibody library and produce human monoclonal antibodies.
经过两步骤的杂交后，构建了转移相关基因的差减文库。
After two steps of hybridization, a subtractive gene bank of metastasis was constructed.
目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
目的：构建大鼠肝细胞热适应差异表达基因消减文库。
AIM: To construct subtractive libraries in association with heat adaptation differential expressed genes.
所构建的消减文库扩增后包含约1000个白色克隆。
在参考其它文献报道的基础上，构建毛竹SSR位点富集文库。
In this experiment, we constructed enriched library that contains SSR loci of Phylbstach- ys pubescens with reference to other reports.
用表面呈现技术构建文库并从中筛选新的生物活性物质，如抗体、受体或疫苗，已成为研究生物新药的捷径之一。
Screening novel products with bioactivities, such as antibodies, receptors and vaccines has become a convenient way to develop novel biopharmaceuticals.
本发明涉及包含抗体替代轻链序列的构建体和文库。
The invention concerns constructs and libraries comprising antibody surrogate light chain sequences.
本发明涉及包含抗体替代轻链序列的构建体和文库。
The invention concerns constructs and libraries comprising antibody surrogate light chain sequences.
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