免疫学方法和探针杂交技术也存在特异性或敏感性的问题。
The technique of immunology and DNA probe hybridization also has the problems of specificity and sensitivity.
目的评价聚合酶链反应探针杂交酶显色方法，检测结核分支杆菌。
Objective To evaluate the value of PCR-probe hybridization-ELISA for detection of mycobacterium tuberculosis(M. tuberculosis).
质粒dna和染色体dna分别与质粒探针和染色体探针杂交出现典型的阳性杂交斑。
Plasmid DNA and chromosomal DNA of the strain CEOI all obtained positive dot-blot to plasmid probe and chromosomal probe correspondingly.
目的：在酶标板上进行DNA探针杂交，评价其在检测临床标本中结核杆菌的应用价值。
Objective: To assess the value of PCRDNA probe in detecting mycobacterium tuberculosis in clinical samples.
目的探讨PCR -荧光双标记探针杂交定量结核杆菌技术对肺结核的诊断价值并进行卫生经济学评价。
Objective To probe the diagnostic value and health economic evaluation of PCR-fluorogenic hybridization assay in pulmonary tuberculosis.
经生物素标记探针杂交确认RANTES基因的整合，用免疫斑点印迹法对重组基因表达水平进行定量测定。
The integration of RANTES gene was confirmed by hybridization with biotin-labelled probes and the recombinant gene expression level was quantitatively detected by immunodot blotting.
结果：SARS阳性样品与寡核苷酸芯片杂交后出现阳性杂交信号，具有不同二级结构的寡核苷酸探针杂交信号强度不一。
Results: Positive SARS samples were detected by oligonucleotide array and fluorescence signal intensity was highly related to the probe's second structure.
方法：采用多重pcr核酸探针杂交技术，对泌尿生殖系统传播疾病(STD)有症状者分泌物，用同一份处理标本与常规pcr法比较。
Methods: with multi-PCR-nucleic-acid probe, We tested the secretion specimens from STD patients with clinical symptoms and compared the results with those of the routine PCR.
P CR技术较体外杂交技术和DNA探针有更高的敏感性。
Compared with biotinylated DNA probes and hybridization technique, PCR has a higher sensitivity.
在分析的过程中对探针的固定和杂交条件进行了探索。
The conditions on probe immobilizing and hybridizing are studied during analysis.
DNA微集阵列技术的关键是探针的制备与样品的标记、杂交。
The key points of the DNA microarray technology include probe preparation, sample labeling and hybridization.
本研究采用地高辛标记的RNA探针，用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。
In this study, the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.
生物芯片的应用是将探针固定于芯片上，利用核酸链间的分子杂交，鉴定dna和蛋白质的一种新技术。
The application of biochips is a new technique which fixes the probes on the biochips to appraise DNA and protein by the way of the molecule crosses between the chains of nucleic acids.
用9个玉米和水稻线粒体基因探针与酶切条带杂交发现，不育系与保持系线粒体DNA的相同大小酶切片段含有相似的顺序。
Homologous sequences of mtDNA were found between MS and maintainer lines by using 9 probes of maize and rice to hybridize with digested mtDNA.
利用该探针与淋巴细胞RNA进行点杂交。
Dot hybridizations of RNA extracted from human lymphocytes were done by using the probe.
寡核苷酸固定过程中，末端氨基修饰没有明显的特异性，但是可以提高被固定探针的杂交容量。
During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides.
通过检测不同探针浓度对探针固定效率及杂交效率的的影响，确定最佳点样浓度。
The optimal spotting concentration was determined through comparing the effect of different capture probe concentration on its covalent fixation and on the hybridization efficiency.
结论与双链探针比较，单链探针的应用可以明显提高RDPCR技术杂交的敏感性及特异性。
Conclusion in comparison with ds probes, the application of ss probes can increase the hybridization sensitivity and specificity of RDPCR.
着丝粒和端粒DNA探针多色荧光原位杂交是一种较为精确的分析微核染色体组成的方法。
Multicolor FISH with centormeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.
利用一定电压下将探针固定在活化的碳糊电极表面的技术，研制成DNA电化学传感器，用该传感器对互补链dna进行杂交检测。
The DNA electrochemical sensor is manufactured by immobilizing ssDNA on the activated surface of CPE in fixed potential. The complementary DNA is detected by hybridization on the this sensor.
应用生物素标记HBVDNA（乙肝病毒脱氧核糖核酸）作探针，对129例肝病患者肝组织作原位杂交研究。
A biotin-labelled DNA specific for hepatitis B virus (HBV) nucleo-tide sequences was hybridized in situ to liver tissue of 129 cases with liver diseases.
分子信标是一种高灵敏度、高特异性的新型荧光核酸探针。它在与互补DNA或RNA靶序列杂交时放出荧光。
Molecular beacon is a kind of high sensitive and selective fluorescence oligonucleotide probes that is producing fluorescence upon hybridizing with their complementary DNA or RNA targets.
探索了探针长度、探针位置等对杂交信号的影响。
The effects of the length and location of probes on hybridization signal were studied.
从各个样本中提取总rna并制备成辅酶r标记的探针和人基因组u 133a芯片杂交，提取和分析数据。
Total RNA from each sample was isolated and biotin-labeled probes were hybridized to the human genome U133A chip, after which the chip was washed and scanned. Data were analyzed using DMT software.
本文介绍了核酸探针和制备技术，标记技术、核酸分子杂交方法及核酸探针的应用状况。
The nucleic acid probe, manufacture and labelling technology, hybrid method of nucleic acid molecule, and the current application status of nucleic acid probe were introduced in this paper.
该文导出了核酸探针在石英晶体微天平电极表面上的杂交反应动力学模型，并通过实验对导出模型进行了验证、计算出相关动力学参数。
The dynamic model of nucleic hybridizing reaction on the surface of the electrode of quartz crystal microbalance(QCM) was deduced, and it was well validated.
方法P CR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交，通过ELISA检测端粒酶活性。
Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA.
我们自己制备的LZF—I探针，能与鱼类基因组DNA中的简单重复序列杂交形成杂交分子。
The oligonucleotide probe LZF-I, which is synthesized by us, can form thehybrid molecules with the simple repetitive DNA of three species of fishes.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
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