共筛选80个RAPD随机引物，其中有27个引物能对DNA扩增并能发现其中抗性基因与感性基因的区别。
RAPD were selected in this study used 80 random primers, 27 primer pairs of DNA can be amplified and can be found resistance genes and gene perceptual difference.
潮霉素抗性浓度的筛选为检测谷子转几丁质酶基因奠定基础。
The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.
结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
为此，致力于发掘作物抗逆相关的新基因，并将之运用到作物抗性品种的筛选与改良上，成了当今一个研究热点之一。
Therefore, to find news gene related with abiotic stresses and to apply them to screen and improve resistance variety become a hot research topic.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
对部分经潮霉素筛选得到的再生植株进行了多次重复P CR检测，发现其中40 %以上的潮霉素抗性植株均表现出较强的阳性反应，初步证明几丁质酶基因已整合到油菜细胞核基因组中。
PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.
应用推荐