结果序列测定初步确定所克隆的是功能性抗体可变区基因。
Results The cloned antibody variable regions genes were approved functional by sequencing.
目的：MCPR 1是我室利用消减杂交技术克隆出来的新基因，本研究利用融合蛋白制备mcpr1的多克隆抗体。
Objective: To prepare polyclonal antibodies of MCPR1, which is a novel Mouse Cleft Palate Relate gene cloned by subtractive hybridization method at our department.
在国内外首次利用抗体探针获得了植物抗病蛋白编码基因的部分序列，为进一步克隆全长的API蛋白基因奠定了基础。
It is the first report of obtaining partial sequences of plant disease resistance gene by using antibody probe in the world, which provides a basis of cloning the whole gene encoding API protein.
目的：表达人免疫相关鸟苷三磷酸酶基因(IRGM) a全长融合蛋白，制备高质量的兔抗人IRGM多克隆抗体。
Aim: To express the whole length fusion protein of human IRGMa and prepare high quality rabbit anti-human immune-related gene guanosine triphosphate (IRGM) polyclonal antibody.
目的：获得抗人视网膜母细胞瘤单克隆抗体轻链可变区基因。
Objective: to acquire the variable region gene of light chain of monoclonal antibody against human retinoblastoma.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
核苷酸序列分析表明所克隆基因分别为抗体轻、重链可变区基因。
The nucleotide sequence analyses indicated that the cloned genes coded the variable light and heavy chain domains from mouse antibodies respectively.
表明可合并组成一段含启动子、起始密码子和NKND四个氨基酸的678个碱基的单克隆抗体M26～32抗原决定簇基因片段。
A combined 678 bp sequence which containing promoter, initiation codon and NKND sequence can be used for further research of target antigen gene of pan species monoclonal antibody M26~32.
目的克隆白癜风患者淋巴细胞中原始配对的自身抗体基因，构建噬菌体抗体库并筛选针对黑素细胞膜抗原的特异性抗体。
AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients' lymphocytes, then construct and screen a human ScFv phage antibody library.
而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
紧密连接蛋白克劳丁-1单克隆抗体能够有效地抑制大多数基因型丙型肝炎病毒感染和自患者分离的高度突变的丙型肝炎病毒准种感染。
The monoclonal antibodies against CLDN1 efficiently inhibited infection by HCV of all major genotypes as well as highly variable HCV quasispecies isolated from individual patients.
研究人员还克隆了576个新HIV抗体，这些抗体源自于少数生殖系免疫球蛋白基因。
They cloned 576 new HIV antibodies, which were derived from a small number of germ-line immunoglobulin genes.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
报道一种克隆免疫脾细胞中抗体可变区基因的新方法。
A novel method in cloning antibody variable genes from immunized spleen cells was described in the report.
因此基因泰克公司开发了一种单克隆抗体，这种蛋白将绑定VEGF而使之失效。
So Genentech developed a monoclonal antibody, a type of protein, that would bind to VEGF and disable it.
应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。
Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing them in recombinant IL-2 and anti CD3McAb.
本发明提供一种CD86人源化单克隆抗体，其相关基因序列和表达系统。
The invention provides a CD86 humanized monoclonal antibody, an associated gene order and an expression system thereof.
AFP的作用均可被抗AFP单克隆抗体所拮抗 。实验结果提示 ，AFP对细胞生长的调节作用可能通过促进这些原癌基因的表达来实现 。
The effects of AFP could be blocked by anti AFP monoclonal antibody. The results indicated that AFP could regulate the growth of HeLa cells through enhancing the expression of these oncogenes.
AFP的作用均可被抗AFP单克隆抗体所拮抗 。实验结果提示 ，AFP对细胞生长的调节作用可能通过促进这些原癌基因的表达来实现 。
The effects of AFP could be blocked by anti AFP monoclonal antibody. The results indicated that AFP could regulate the growth of HeLa cells through enhancing the expression of these oncogenes.
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