结论错配pcr结合噬菌体展示技术是提高单链抗体亲和力的一种有效且简便的手段。
Conclusion Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
该方法体外模拟了抗体亲和力成熟的过程，使得到的抗体不但可以完全的人源化，而且可以具有较高的亲和力。
Because this method was to simulate the process of antibody maturation in vivo, the obtained antibodies was not only fully humanized but also with relatively high affinity.
目的建立一种测定噬菌体抗体相对亲和力的方法。
Objective To establish a method for the measurement of relative affinity of phage antibodies.
最后应用克隆算法得出了优化的故障恢复策略，选择亲和力高的抗体并进行克隆。
Clonal algorithm is adopted to educe optimized service restoration strategy, through selecting and clone antibody with high affinity.
而抗体介导的免疫治疗的关键是靶抗原的特异性强，抗体的免疫原性低及亲和力高等。
The key of antibody-mediated immunological therapy is strong specificity of target antigen, low immunogenicity, high affinity of antibody and so on.
结论抗体抗原亲和力随免疫次数的增加趋向饱和，BSA的线性表位为优势表位，随免疫次数的增加比重逐渐增强。
Conclusion With the increase of the immunization times, the affinity of antibody come to mature and the proportion of dominant linear epitopes of BSA became higher.
除了我们讨论的的高亲和力抗体外，我们的机体也产生许多抵抗感染的低亲和力抗体。
In addition to the high affinity antibodies we're talking about, our bodies also produce a lot of low affinity antibodies for mopping up infection.
文章评述了这一领域的最新进展，同时也讨论了工程抗体的免疫原性和亲和力。
This review summarized recent developments in this field, also discussed the immunogenicity and affinity of engineered antibodies.
该磺胺二甲基嘧啶抗原免疫动物可以制备出具有更高亲和力和特异性的抗体。
The sulfamethazine antigen immune animal can prepare the antibody with higher appetency and specificity.
记忆单元保存抗体群中亲和力较高的抗体。
The memory cell keeps better antibodies of the antibody population.
通过试管法和玻片法检测抗a抗体的效价和亲和力。
Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.
结论：硫氰酸盐洗脱法用于噬菌体抗体的筛选有助于成功获得期望的高亲和力抗体。
Conclusion:Thiocyanate elution is a powerful and effective method to help obtain the Phabs with high affinity from phage antibody library.
本发明包括抗体，该抗体通过以高度亲和力及特异性结合天然人类TF而提供优异抗凝血活性。
The invention includes antibodies that provide superior anti-coagulant activity by binding native human TF with high affinity and specificity.
本发明提供以高亲和力特异性结合O8E的分离的单克隆抗体，特 别是人单克隆抗体。
The present disclosure provides isolated monoclonal antibodies, particularly human monoclonal antibodies that specifically bind to O8E with high affinity.
噬菌体展示技术是一种制备高亲和力抗体的有力工具，抗体库技术则为筛选高亲和力抗体提供了良好的平台。
Phage displayed technology has become a powerful tool in preparing high affinity antibodies, and antibody library technology paves a way for obtaining high affinity antibodies.
本发明提供以高亲和力特异性结合IRTA - 2的分离的单克隆抗体，特别是人单克隆抗体。
The present invention provides isolated monoclonal antibodies, particularly human monoclonal antibodies that specifically bind to IRTA-2 with high affinity.
亲和力是反映抗体特性的一个重要指标，也是抗体研究中经常需要测定的参数，在抗体的筛选、鉴定乃至其功能和应用研究等方面都涉及到亲和力的问题。
Objective To establish a method for the measurement of relative affinity of phage antibodies. Methods The relative affinities of 5 phage antibodies were measured by ELISA using thiocyanate elution.
亲和力是反映抗体特性的一个重要指标，也是抗体研究中经常需要测定的参数，在抗体的筛选、鉴定乃至其功能和应用研究等方面都涉及到亲和力的问题。
Objective To establish a method for the measurement of relative affinity of phage antibodies. Methods The relative affinities of 5 phage antibodies were measured by ELISA using thiocyanate elution.
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