组：单纯成纤维细胞培养组。
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
在细胞单层培养系统的基础上，将瘢痕组织来源的成纤维细胞培养在胶原凝胶中，形成了瘢痕成纤维细胞的三维培养系统。
Based on the monolayer cell culture system, we resuspended the scar-derived fibroblasts in a solution of polymerizing collagen to develop a "three-dimensional" cell culture system.
目的在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
目的和方法：利用细胞培养和RT P CR技术研究新生大鼠心脏成纤维细胞增加心肌细胞搏动频率的作用。
Aim and Methods: To study the effect of ventricular fibroblasts increase beating rate on cultured neonatal rat cardiomyocytes by using cell culture and RTPCR technique.
方法：采用经具核梭杆菌LPS刺激的单核细胞培养上清作用于体外培养的人牙髓细胞，通过OD值测定，观察单核细胞培养上清对人牙髓成纤维细胞碱性磷酸酶（ALP）活性的影响。
Methods:The culture supernatant of monocytes stimulated with Fn LPS was applied to human dental pulp cell in vitro, and activity of ALP in human dental pulp fibroblast was monitored by OD test.
方法：取新生icr小鼠皮肤细胞培养成纤维细胞。
Methods: Mouse skin fibroblasts were obtained from newborn ICR mice and propagated in vitro.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
方法采用体外细胞培养技术进行皮肤成纤维细胞的分离、培养、传代、冻存、复苏及鉴定。
Methods Cell culture in vitro technique was used, including isolation, culture, subculture, frozen storage, recovery and identification of human fibroblasts, in this study.
应用推荐