方法应用PCR为基础的微卫星多态性分析技术。
Methods PCR-based microsatellite polymorphism analysis technique was used.
目的调查广东地区汉族人群MICA基因第5外显子和MICB基因第1内含子微卫星多态性分布。
Objective To investigate the genetic polymorphism of microsatellite in the exon 5 of MICA gene and the intron 1 of MICB gene in Guangdong Han population.
方法:采用PCR及微卫星多态性技术,对65例肝癌1号染色体上28个微卫星标志位点杂合性缺失进行检测。
Methods: LoH of chromosome 1 were detected by PCR based microsatellite polymorphism analysis technique using 28 pairs of microsatellite markers primers in 65 HCC.
微卫星DNA因具有长度多态性这一特点,而被广泛应用于个体的亲子鉴定。
Microsatellite DNA is characterized by its length polymorphism, so it is widely used in the individual paternity.
结论中国汉族雄激素受体基因微卫星CAG多态性对PCOS高雄激素血症的形成无明显关系,不是PCOS的主要致病因素。
Conclusion: androgen receptor gene CAG microsatellite polymorphism exists in Chinese women does not significantly relate with pathogenesis of hyperandrogenism and is not the main cause of PCOS.
利用微卫星DNA在萨福克羊群体中的多态性预测绵羊群体的肉用性能。
The meat performance of Suffolk sheep population was forecasted by polymorphism of micro-satellite DNA.
目的研究近交系小鼠微卫星位点的遗传标记多态性及其意义。
To study the genetic marker polymorphisms and role of microsatellite of inbred strain mice.
目的探讨雄激素受体基因微卫星CAG多态性与多囊卵巢综合征(PCOS)患者高雄激素血症的关系。
Objective: To explore the relationship between CAG microsatellite polymorphism of androgen receptor gene and hyperandrogenism of polycystic ovary syndrome (PCOS).
方法:荧光标记的多态性微卫星引物d10s1265与83例结直肠癌的肿瘤和正常组织进行PCR反应。
Methods: D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR.
短串联重复(STR)是Y染色体NRY区的多态性遗传标记的一种,又称微卫星。
Short tandem repeat ( STR) is one of the genetic polymorphic markers within the NRY region in Chromosome Y, which is also called microsatellite.
微卫星,即简单重复序列(SSR,1 ~6个碱基单元),广泛存在于基因组中,具有丰富的多态性。
Microsatellites, tandem Simple Sequence Repeats (SSR, 1 ~ 6 bp motif) are abundant across the genome and show high levels of polymorphism.
结果表明,有13对引物能获得稳定的特异性条带(占总数的50%),其中有6个微卫星位点具有多态性(占总数的23.1%)。
Electrophoresis analysis showed that 13 pairs of primers produced stable and specific bands(50%), and, of which 6 were polymorphic(23.1%).
实验结果表明,所选用的微卫星标记表现出丰富的多态性,可成功地应用于丝羽乌骨鸡的遗传分析。
The results suggested that the eighteen microsatellite markers could be successfully applied to the silkies genetic analysis.
方法对来自中国不同地域的松杨栅锈菌的5个生理小种11个菌系核糖体its序列和微卫星序列多态性进行了研究。
ITS sequences and ISSR markers based on 11 isolates of 5 RACES collected from different parts in China were studied.
多态性缺失是微卫星不稳定性的表现形式。
Loss of polymorphism is a special form of expression of microsatellite instability.
结论:研究人群与文献报道的白种人群相比,这3个微卫星DNA位点的多态性在种族和地区分布上有差异。
Conclusion: in contrast to the information about Caucasian, the polymorphism of these 3 microsatellite DNA differs from race to race, and region to region.
结果表明,共检测到174个等位基因,23个座位都呈现出多态性,23个微卫星座位的等位基因数在3 ~12个,平均等位基因数为7.5个。
A total of 174 alleles were detected across the 23 microsatellite loci. All the loci were polymorphic and the number of alleles varied from 3 to 12, and the mean number was 7.5 alleles per locus.
目的:研究脆性组氨酸三联体(FHIT)基因微卫星D3S1234和D3S1300长度多态性与肺癌的关系。
AIM: To analyze the relationship between lung cancer and length polymorphisms of microsatellite D3S1234 and D3S1300 located on fragile histidine triad (FHIT) gene.
利用微卫星分子标记,对湖南目前推广的6个杂交稻组合及其亲本之间的多态性进行了研究。
Six hybrid rice combinations and their parents were used to screen their polymorphic patterns using microsatellite markers.
利用微卫星分子标记,对湖南目前推广的6个杂交稻组合及其亲本之间的多态性进行了研究。
Six hybrid rice combinations and their parents were used to screen their polymorphic patterns using microsatellite markers.
应用推荐