腺病毒载体是继逆转录病毒载体后在基因治疗、基因免疫等方面应用开发得较早且较成熟的一种基因载体。
Adenovirus vector is an earlier and maturer genetic carrier in the respect of gene therapy and genetic immunization after retroviral vector.
目的探索逆转录病毒载体在基因治疗乙型肝炎中的应用。
Objective To investigate the effectivness of recombined retrovirus vector in gene therapy.
目前应用的病毒载体主要有逆转录病毒载体、腺病毒载体、腺病毒相关病毒、慢病毒载体、单纯疱疹病毒载体等。
At present, the mainly applied virus vectors are retroviral vector, adenovirus vector adenovirus-associated virus slow virus vector, herpes simplex virus vector and so on.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。
The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.
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