方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
亚硫酸氢钠测序检测表达有差异的基因调节序列的甲基化状态。
Bisulfite sequencing was used to determine the methylation status of the regulatory sequence in which gene the expression are significantly difference.
结果显示，VEGFASODN对U937细胞的VEGF的表达有明显的抑制作用，与错义序列组和空白对照组相比有显著差异（P<0 .0 5 ） ；
The result showed that VEGF ASODN had obviously inhibitive effect on expression of VEGF in U937 cell, as compared with scrambled sequence and negative control (P<0.05).
为了在基因表达水平上研究其耐盐机制，本实验室制作了盐藻在高渗震荡下的表达谱基因芯片，从中筛选到了一系列高渗震荡下差异表达的序列。
In order to study the salt tolerance mechanism at the expression level, our laboratory made the expression gene chips of Dunalliena salina after high salt shock.
对两个抗病品种表达量上调的片段进行回收，总共得到了13条差异序列，其中有6条差异序列在两个品种中均有表达。
The up-regulated bands of the two resistance varieties were picked out and cloned, 13 TDFs were gained in all, and 6 TDFs are both in the two varieties.
对两个抗病品种表达量上调的片段进行回收，总共得到了13条差异序列，其中有6条差异序列在两个品种中均有表达。
The up-regulated bands of the two resistance varieties were picked out and cloned, 13 TDFs were gained in all, and 6 TDFs are both in the two varieties.
应用推荐