方法：应用深低温保存1～60月的健康人尸角膜为真菌性角膜炎10例、10眼和病毒性角膜炎4例、4眼行穿透性角膜移植术。
Method:10 cases(10 eyes)of fungal keratitis and 4 cases(4 eyes) of viral keratitis were performed penetrating keratoplasty with health human cadaver corneas cryopreserved 1 to 60 months.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
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