用融合蛋白表达系统对该半分子进行大肠杆菌表达。
Then, a fusion protein expression system was used to express the TNF half molecule.
目的构建可用于大肠杆菌表达系统的含抗冻蛋白基因afp的表达载体。
Objective: to obtain the procaryotic expression vector containing the antifreeze protein gene AFP.
目的：探索重组人胎盘抗凝蛋白变体大肠杆菌表达菌株的最适表达条件。
Objective the purpose of this study is to optimize the different parameters in the expression of recombinant human placental anticoagulant protein derivative expressing e.
对大肠杆菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.
结论成功用大肠杆菌表达系统表达创伤弧菌溶细胞素并对其纯化、复性条件进行优化。
These results indicate that the cytolysin gene from V. vulnificus successfully express in the E. coli system.
首先，我们在第11章中知道了关于大肠杆菌表达的蛋白产物的纯化工艺优化和特性研究。
First, we learn in Chapter 11 about process optimization and characterization studies for the purification of an E. coli-expressed protein product.
本文报道了在克隆了小鼠酪氨酸酶基因的基础上，在大肠杆菌表达体系中诱导表达野生型和变异型酪氨酸酶的结果。
Here we reported the cloning of the gene from murine tyrosinase, the expression of wild tyrosinase and variants in E. coli.
以大肠杆菌表达的传染性法氏囊病病毒(IBDV)衣壳蛋白vp2致敏绵羊红细胞，建立间接血凝试验(IHA)。
An indirect hemagglutination assay (IHA) was set up using sheep erythrocytes sensitized with capsid protein VP2 of infectious bursal disease virus (IBDV) which were expressed in Escherichia coli.
结论利用大肠杆菌表达的HEV重组抗原建立了双抗原夹心法elisa，并可同时用于不同动物的抗hev抗体检测。
Conclusion a double antigen sandwich ELISA have been established using recombinant antigens expressed in E. coli., and can be used to detect anti HEV in different kinds of animals.
目的：克隆长双歧杆菌ncc 2705株果糖结合蛋白bl 0033的基因，利用大肠杆菌表达GST -BL 0033融合蛋白并纯化。
Objective: to clone the gene of fructose binding protein BL0033 from Bifidobacterium longum NCC2705, and express and purify fusion protein GST-BL0033 in e.
创伤弧菌中的溶细胞素在大肠杆菌系统中成功表达。
The cytolysin gene from V. vulnificus successfully express in the E. coli system.
将纯化的在大肠杆菌中表达的鹦鹉热衣原体（Cps）的重组主要外膜蛋白（MOMP）与油佐剂混合。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci (Cps) expressed in E. coli was mixed with oil adjuvant.
助手读了剪贴板上的文字，“共同表达发酵单胞菌属PDC和ADH基因的大肠杆菌菌株的乙醇生产”。
“Ethanol Production by Escherichia Coli Strains Co-Expressing Zymomonas PDC and ADH Genes, ” said the aide, reading from a clipboard.
他们利用7个大肠杆菌菌株群，每个都加入了能表达出不同颜色荧光蛋白的基因。
The new scheme replaces the fuse with seven colonies of Escherichia coli bacteria, each given a gene for a different fluorescent protein.
以EDDIE为融合蛋白是在大肠杆菌中高效表达抗菌肽的一种好方法。
This method provides an excellent way for high expression of antimicrobial peptides when fused with EDDIE.
该抗血清不但能识别来源于大肠杆菌的抗原，还能检测真核细胞内转染后表达的IRF-7。
The antisera can recognize not only recombinant IRF-7 expressed in E. coli, but also transfected IRF-7 in mammalian cells.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective To construct recombinant plasmid for expression of chymopapain in E.
目的：构建丙型肝炎病毒(HCV)全长及3种不同缺失突变的核心蛋白基因的原核表达载体，并在大肠杆菌中表达。
AIM: to construct the recombinant plasmids expressing full-length HCV core protein gene and 3 different deletion mutated hepatitis core protein genes and to express them in E. coli.
结论：成功地克隆了人THANK基因，并在大肠杆菌中获得表达，为其功能的研究打下基础。
Conclusion: Human THANK gene was cloned and expressed successfully, which provides a base of further research of THANK gene.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
目的：合成胆汁三烯结合蛋白(BBP)基因并在大肠杆菌中表达，获得重组BBP纯化制品。
Objective: To synthesize bilin binding protein (BBP) gene sequence, express BBP efficiently in Escherichia coli and purify the recombinant protein.
目的构建人细胞色素P450 4f 2基因野生型、V81G和V433 M突变型表达载体，在大肠杆菌中表达并纯化出CYP4F2蛋白。
Objective to construct the expression vectors of human cytochrome P450 (CYP) 4f2 wild type, V81G and V433M Variants and express and purify CYP4F2 protein in e.
利用鹦鹉热衣原体重组主要外膜蛋白（MOMP）在大肠杆菌中的表达产物，纯化后与白油佐剂乳化成疫苗。
The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci(Cps)expressed in E. coli was mixed with oil adjuvant and manufactured as MOMP-vaccine.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
重组人脑源性神经营养因子（BDNF）在大肠杆菌中表达，以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96％。
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
在大肠杆菌中表达量约占细菌总蛋白15 % ，在昆虫细胞中表达量约占细胞总蛋白10 % 。
The expressed target protein accumulated up to about 15% and 10% of the total cellular proteins, respectively.
目的构建大肠杆菌K99菌毛表达载体。
结果：成功克隆了CAT的基因片段，并在大肠杆菌中得到其融合蛋白的表达。
Results: The CAT gene was cloned correctly and it's fusion protein was expressed in E.
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