基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。
Gene transfer vector is the important ingredient in gene therapy, which includes virus vector and non-virus vector.
腺病毒作为一种重要的基因转移载体，在应用中存在着靶向性差的问题。
As an important gene transfer vector, adenovirus has the deficiency of poor targeting.
基因转移载体是基因治疗的重要组成部分，包括病毒载体和非病毒载体。
Gene transfer vector is the important ingredient in gene therapy, which includes virus vector and nan-vires vector.
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。
Additionally, baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non-reproduce vector.
逆转录病毒介导的基因转移技术要过渡到临床应用，主要解决如何使病毒载体具有高滴度而不具有复制活性。
In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses.
重组病毒载体因其自然感染途径而成为有效的基因转移手段。
Recombinant viral vectors have become effective tools for gene transfer by means of their natural infective ways.
目的构建含小鼠白细胞介素12双亚基及新霉素磷酸转移酶基因多顺反子逆转录病毒载体，并观察其在小鼠肝癌细胞中的表达。
Objective to construct a polycistronic retroviral vector containing both subunits cDNA of murine IL 12 and NeoR gene and investigate their expressions in murine hepatoma cells.
将外源的基因导入生物细胞内必须借助一定的技术方法或载体，目前基因转移的方法分为生物学方法、物理方法和化学方法。
The foreign gene into the biological cells must use a certain technology or approach vector, the current methods of gene transfer into biological methods, methods of physical and chemical methods.
目的构建人胰岛素样生长因子- 1的逆转录表达载体，建立逆病毒介导的IGF - 1基因转移系统。
Objective to construct a retroviral mediated expression system of human insulin-like growth factor-1 (IGF-1), and determine whether bone marrow stromal cells (BMSCs) can be infected by retrovirus.
随着对病毒载体的不断改造完善，提高其基因转移效率和安全性，病毒载体将在基因治疗中继续发挥重要作用。
With the increasing reform and consummation of virus vector, it can play its important role continuously in gene therapy by promoting its efficacy and safty in gene transfer.
对角膜内皮基因转移技术和载体的研究现状，及未来载体的研究方向进行综述。
The review summarized the current studies of gene transfer to corneal endothelium and vectors that are been using. In addition, the review indicates the study prospect for future vectors.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
本发明还涉及人源化改造的基因突变技 术和去除GFP的腺病毒转移载体及去除GFP的同源重组腺病毒载体。
The invention also relates to a human reforming gene mutation technique, adenovirus transition carrier removing GFP and homologous recombinant adenovirus carrier.
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