主要应用于新基因发现、基因表达分析、基因突变及多态性分析、基因测序等。
The main application is in discovering new genes, analysis of gene expression, gene mutation, polymorphism and genetic sequence test.
目前,该技术已用于基因表达谱分析、新基因发现、基因突变、基因多态性分析、药物筛选和基因测序及疾病的诊断。
By the mean time, the technique have reported in the gene expression analysis, new gene discovery, gene mutation detection, SNP analysis, DNA re-sequence and diagnosis.
方法:应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列,设计全新的抗原表位编码基因序列,构建表达载体并在大肠杆菌中表达该基因。
Methods: By analyzing the antigen epitope of SARS-CoV s protein with antigenic peptides prediction software, a new gene was designed, and its expression vector was constructed.
通过对P25基因启动子的活性分析,尤其是对PS GF和BMFA调控元件的功能分析,有利于进一步了解P 25基因表达调控的精细机制。
Analysis of P25 promoter activity, especially functional analysis of PSGF and BMFA regulatory elements, could be helpful in further understanding the mechanism of P25 expression and fine regulation.
通过对P25基因启动子的活性分析,尤其是对PS GF和BMFA调控元件的功能分析,有利于进一步了解P 25基因表达调控的精细机制。
Analysis of P25 promoter activity, especially functional analysis of PSGF and BMFA regulatory elements, could be helpful in further understanding the mechanism of P25 expression and fine regulation.
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