有各种逆转录酶聚合酶链反应(扩增核糖核酸基因组RT–PCR)检测试验方法，但灵敏度各不相同。
Various reverse transcriptase–polymerase chain reaction (RT–PCR) methods are available but are of variable sensitivity.
各种作物基因组大小的差异可能由于各个区段内基因间重复顺序扩增的程度不同所致。
The differences of genome size among cereals may be due to different degrees of repetitive sequence amplification in intergenic regions in different species.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
该文以DNA PCR扩增的方法，从拟南芥基因组DNA中克隆出NPR1基因，通过序列分析，所克隆的NPR1 基因与报道的基因序列完全一致。
The NPR1 gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPR1 gene was the same as the published sequence.
利用ISSR标记对15份葡萄材料进行了基因组多态性分析，从60条引物中筛选出15条用于葡萄的ISSR扩增。
Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. 15 primers selected from 60 primers were used for ISSR amplification.
改进了从鲸类陈旧骨骼标本中进行基因组dna提取和PCR扩增的方法。
The method to extract genome DNA from old skeletal specimens of cetaceans was improved and the PCR amplification reaction was optimized.
结论以人外周血单个核细胞基因组dna为模板，扩增人CD 14基因的方法是一种获取CD 14基因简捷、有效的方法。
Conclusion It is a simple and effective method to obtain human CD14 gene from genome DNA of human mononuclear cell in peripheral blood.
方法：从独活药材中提取基因组dna，运用RAPD(随机扩增多态性DNA)技术对其进行鉴别。
Methods: the genome DNA was distilled from Tetrandra Root, and differentiate it by RAPD (the random amplification polymorphism DNA).
抽提外周血基因组dna，对DAX1基因2个外显子(外显子1和2)的PCR扩增产物进行测序分析；无突变者行SF 1基因(外显子2 ~ 7)突变筛查。
Genome DNA was extracted from peripheral blood, exon 1 and exon 2 of DAX1 gene were amplified by PCR for sequencing, and mutation screening of SF1 gene (exon 2-7) was conducted.
从羊痘病毒基因组中寻找并扩增羊白细胞介素-18结合蛋白（IL-18BP）基因。
Some virus genome of vaccinia subgenus in poxvirus encoded IL-18BP gene, which can block IL-18 activity but no sequence similarity to membrane IL-18 receptors.
方法从纳豆芽胞杆菌中分离纯化基因组DNA ，作为模板通过PCR扩增纳豆激酶原基因。
Methods Chromosome DNA was isolated from Bacillus natto . The pro nattokinase gene was then amplified from chromosome by PCR.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
为了排除基因组dna中假基因的干扰，利用扩增阻滞突变系统，成功地分析了一个肾上腺脑白质营养不良(R 6 17g突变)家系成员的基因型。
To avoid the interference, genomic DNA from the family members with an adrenoleukodystrophy gene mutation (R617G mutation) was analyzed by amplification refractory mutation system.
目的探索随机扩增多态性DNA（RAPD）技术对大陆光亮钉螺遗传多态性研究的可行性，以获得不同地区光壳钉螺基因组DNA水平上的差异信息。
Objective Using RAPD technique to study genetic diversity of smooth-shelled Oncomelania hu- pensis in China and to gain more information on genome DNA level.
使用多重连接依赖式探针扩增（MLPA），我们在三个家族中检测两个大的基因组重排，检测21和22外显子和整条BRCA1基因上的一个罕见缺失。
Using MLPA analysis, we detected two large genomic rearrangements in three families, a deletion of exons 21 and 22, and a rare deletion of a whole BRCA1 gene.
为研究IBV变异株的基因组及其变异特征，本实验通过基因扩增拼接的方法获得了肾病变型毒株JH06111的全基因组序列。
To study the genomic characteristics of IBV variant isolate, we obtained the full genome sequences of nephropathogenic JH06111 by amplifying, cloning and sequencing of its cDNA.
利用随机引物对基因组DNA扩增结果表明，NIL -PL0 1和NIL -APL0 1之间多态性差异较小，遗传同质性达98.6 % ，该材料正用于筛选与无花瓣性状紧密连锁的分子标记。
The genomic DNA PCR indicated that the genetic homogeneity between NIL-PL01 and NIL-APL01 reached 98.6%, which were being used in screening the molecular marker linked to apetalous trait.
结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。
CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.
结果7种硬蜱基因组随机扩增产物均有各自独特的DNA条带，种间的平均遗传距离为071。
Results the amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 071.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
昆虫基因组大小是由于基因组各种重复序列在扩增、缺失和分化过程中所致的数量差异造成的。
The difference of genome size between different insects is due to varieties of duplication of the genome sequence in the amplification, deletion and differentiation.
PCR扩增结果初步表明外源基因已整合到棉花的基因组中。
PCR results showed that there were transgenes in the cotton genome.
扩增结果初步说明外源基因已整合到植物基因组中。
The result of PCR indicated that the foreign-gene had been integrated into the plant genome.
扩增结果初步说明外源基因已整合到植物基因组中。
The result of PCR indicated that the foreign-gene had been integrated into the plant genome.
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