结论：成功构建小鼠emsp1基因打靶载体。
Conclusion The mouse EMSP1 gene targeting vector is constructed successfully.
Ko正在研究利用目前用于猪，牛和其他动物的程序给克隆狗进行基因打靶。
Ko is working to adapt a procedure used so far in pigs, cows and other animals to target genes in cloned dogs.
本文就基因打靶技术及其在人类疾病动物模型研究中的应用做一介绍。
Gene targeting technology and its application in research of animal model of human disease were briefed in this paper.
简要阐述了基因打靶的概念和基本环节，系统介绍了筛选方法和基因打靶的策略。
Briefly introducing concepts and the primary technical procedure of gene targeting, and systematically introducing selective methods and gene targeting strategies.
简要阐述了基因打靶的概念和基本环节，系统介绍了筛选方法和基因打靶的策略。
Briefly illustrating concepts and the primary technical procedure of gene targeting, and systematically introducing selective methods and gene targeting strategies.
基因打靶技术是建立在胚胎干细胞和同源重组技术之上，可对基因组进行定点修饰的实验方法。
Established upon the embryo stem cell technique and homologous recombination, gene targeting has been widely used in the genome specific manipulation.
核移植克隆猪技术的出现为生产转基因猪提供了一种有效的方法，并且是目前生产基因打靶猪的惟一方法。
Pig cloning provides an efficient method for producing transgenic pigs, further more, it is the only approach which has been used to produce gene-targeted pigs so far.
这种基因敲除程序现在已经被普遍用于小鼠和大鼠，3名研究人员因为发明了这种“基因打靶”技术获得了2007年的诺贝尔奖。
These knockout procedures are now commonly done in mice and rats, and three researchers earned a Nobel prize in 2007 for developing this method, called "gene targeting".
基因打靶技术在小鼠es细胞上被广泛应用，但由于大家畜很难制备向生殖系分化的ES细胞系，所以基因打靶的应用变得非常困难。
Gene targeting technology has been widely applied to mouse es cell, but more difficult to livestock species because es cell of livestock species could not differentiate into germ cell line.
结论通过构建基因打靶载体 ，可有目的地敲除弓形虫膜抗原基因 ，为进一步研究弓形虫侵入机制 ，探讨弓形虫病防治提供可行的方法。
These results demonstrate the utility of the gene targeting approach in the study to investigate the gene function of T. gondii. and the mechanism by which this parasite invades to the host cells.
结论通过构建基因打靶载体 ，可有目的地敲除弓形虫膜抗原基因 ，为进一步研究弓形虫侵入机制 ，探讨弓形虫病防治提供可行的方法。
These results demonstrate the utility of the gene targeting approach in the study to investigate the gene function of T. gondii. and the mechanism by which this parasite invades to the host cells.
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