用退火控制引物(acp)的差异筛选分析法对该样本进行候选基因的初筛。
The pooled samples were initially screened for candidate genes for narcolepsy by differential display analysis using annealing control primers (ACP).
方法4例因献血感染HCV病人的系列血标本，用特异性引物扩增5'-NCR和核心区基因并进行测序。
Methods Serial sera were collected from 4 plasma-donors with HCV infection. 5' - NCR and core gene were amplified, sequenced and analyzed using software.
用双引物法对GI基因进行体外定点突变，构建了GI突变体g 138p。
The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.
经d NA序列分析表明所获基因含有全部前导序列，其成熟蛋白编码部分与从第一骨架区引物所克隆的序列相符。
DNA sequences analysis indicated that the cloned genes included the whole leader sequences and the mature Ig protein encoding regions.
共筛选80个RAPD随机引物，其中有27个引物能对DNA扩增并能发现其中抗性基因与感性基因的区别。
RAPD were selected in this study used 80 random primers, 27 primer pairs of DNA can be amplified and can be found resistance genes and gene perceptual difference.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
本文采用人sry基因的一对引物，通过PCR扩增获得了雄性大熊猫SRY基因片段。
Using human SRY gene primers, the gene fragment of giant panda homologous to human SRY was amplified by PCR.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
PRIMER3被用来提取来自感兴趣的基因的最佳引物。
PRIMER3 was used to extract optimal primers from a gene of interest.
根据计算机克隆的ZNF322基因序列设计引物，从人类胚胎心脏文库中克隆了ZNF322基因。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
根据人的GAPDH基因和毛冠鹿的钾通道基因设计引物。
We designed and synthesized the primers of the human GAPDH gene and the Elaphodus cephalophus potassium channel gene.
选用34个随机引物对T4代高蛋白小麦和对照的基因组d NA进行分析。
Analyzed the genome DNA of high-protein wheat of T4 and CK by 34 random primers using RAPD.
SOX基因兼并引物延伸发现了更多的SOX基因位座，并进一步证实该家族基因在基因组中是散在存在的。
SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes.
这31条多态性引物和所检测到的93个多态性位点初步为草鱼基因组的多态性分析提供了可靠的分析指标体系。
Both the 93 polymorphic loci and the 31 polymorphic primers might provide a reliable molecular marker system for grass carp genomic DNA polymorphism analysis.
每对引物扩增的等位基因数在3 ~13个，平均每个位点有5.6个等位基因，显示了较高的多态性。
The allele Numbers for each primer ranged 3 to 13, and a mean of 5.6 alleles were found for each locus, which indicated a higher polymorphism.
利用ISSR标记对15份葡萄材料进行了基因组多态性分析，从60条引物中筛选出15条用于葡萄的ISSR扩增。
Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. 15 primers selected from 60 primers were used for ISSR amplification.
根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。
Based on the reported genomic RNA sequence of PLRV, two specific primers were synthesized.
根据菌属基因序列设计PCR引物，提取基因组dna进行PCR快速检测，然后进行测序比对。
According to the bacteria gene sequence, we also designed PCR primer, and collected genome DNA for PCR rapid test, then tested and contrasted sequence.
图1抗性基因STV11引物设计策略红色表示引物结合位点，蓝色表示序列差异位点。
Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.
结果用鞭毛蛋白基因作引物的PCR方法具有高灵敏度，其他3种对照结果阴性。
ResultsThe PCR by specific flagellin gene primer has high sensitivity. All of 3 controls were negative.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。
Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.
目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。
Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.
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