实验表明,用基因工程表达的多肽筛选抗原表位的方法是可行的。
The method based on expressed peptide for mapping epitope on viral prot…
启动子对外源基因的表达水平影响很大,是基因工程表达载体的重要元件。
Promoter makes a great influence on the expression levels of exogenous protein as an important component of expression vector in genetic engineering.
综述了与杆状病毒DNA复制相关基因的研究进展。杆状病毒表达系统是最重要的四大基因工程表达系统之一,杆状病毒还具有作为生物杀虫剂的潜能。
Baculovirus expression system (BES) is one of the most important expression systems. Baculovirus also has potential ability as pesticide. DNA replication is the central step in its life cycle.
方法构建基因工程菌,利用甲醇诱导表达。
MethodThe genetic engineering bacteria was constructed, and induced by methanol.
本论文应用基因工程的手段克隆、表达并纯化了人的肝再生增强因子基因。
This dissertation USES the gene engineering method to clone, express and purifies the human ALR gene.
诱导型基因表达系统已成为基因工程和病理学研究的重要工具。
Inducible gene expression system has become an important tool for genetic engineering and pathophysiologic studies.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒,稳定高效地获得基因工程产品心钠素。
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
纤维素酶基因工程的主要难题是如何实现不同组分的有效表达和如何提高表达量。
The main problem in genetic engineering of cellulase is how to achieve effective expressions of different components and how to improve the expression level.
目的:优化融合表达抑肽酶基因工程菌的发酵条件,分离纯化抑肽酶。
Aim: to optimize the fermentation conditions of engineering bacteria expressing fusion proteins composed of aprotinin and to purify aprotinin.
指出,只有进一步深入研究植物基因的结构和表达,深入研究植物组织培养技术,植物基因工程才可能最终进入实际应用。
It arrives at the conclusion that the plant and genetic engineering could not be applied to practice until the structure expression of genes, the plant tissue cul...
结论已成功地在大肠杆菌中表达了IL - 10,为进一步纯化和制备IL - 10的基因工程药物打下了基础。
Conclusion IL-10 was successfully expressed in E. coli. It laid a foundation of further purification and preparation of genetic engineering drug of IL-10.
目的构建人源性抗hfrs病毒基因工程抗体基因的表达载体,为其进一步在真核细胞中表达奠定基础。
Aim to construct anti HFRS virus human engineering antibody expression vectors and then to express it in eukaryotic cells.
外源基因在该基因工程菌中得到了高效表达。
The gene was highly expressed in the engineering bacterial strain.
结果:建立了新基因HBRP稳定表达的基因工程细胞系。
Results: the stable expression cell system of the novel gene HBRP was constructed.
汪静。李官成。童永清鼻咽癌人源抗独特型基因工程抗体的原核表达及鉴定。
Wang J. Li GC Expression, purification and identification of the human anti-idiotypic single chain antibodies against nasopharyngeal carcinoma in E. coli.
相应地将构建的表达质粒导入变铅青链霉菌TK24中获得五株基因工程菌株。
These plasmids were introduced into S. lividans TK24, respectively and five genetic engineering strains were constructed.
为了能寻求一种切实可行及表达高活性小分子ngf的生产途径,我们通过基因工程和细胞工程技术设计了两种NGF的体外重组方案。
To look for a good method to producing NGF which has low molecular weight, we designed two projects which are carried out through gene cloning and cell engineering techniques to provide the problem.
测定了猪生长激素基因工程菌不同表达效率时细菌总蛋白的含量以及包涵体中重组猪生长激素占包涵体总蛋白的比值。
We determined the content of the total bacterial proteins and the proportion of recombinant porcine growth hormone(rpGH)in inclusion bodies(IBs)in the cultures of genetically engineered rpGH E.
HEV结构区ORF2蛋白在甲醇营养型酵母中的成功表达,以及初步纯化得到的具有强免疫学活性的重组蛋白,为研制新型戊型肝炎基因工程疫苗奠定了基础。
The successful expression of HEV ORF2 protein in p. Pastoris and the production of recombinant protein provides basis for genetically engineered vaccine development of hepatitis E.
本发明涉及生物技术中的基因工程领域,公开了一种含绿色荧光蛋白基因的植物转基因表达载体,以及其构建方法和应用。
The invention discloses a plant transgene expression carrier containing green fluorescent albumen gene, its method for constructing the same and use in genetic engineering field of biotechnology.
综述了不同蜘蛛丝蛋白的模块结构特征及与其功能的关系,扼要介绍了目前利用各种基因工程方法表达重组蜘蛛丝蛋白的研究进展。
This mini-review summarized the current understanding of different spider silk proteins in respect of the structural modules and their functional correlations, and the ma.
目的构建生产辅酶Q 10的大肠杆菌基因工程菌并研究相关酶的表达情况。
OBJECTIVE To construct Escherichia coli producing coenzyme Q10 and investigate the decaprenyl diphosphate synthase expression.
该启动子可以驱动外源基因在转基因植物中热激诱导表达,可以代替35s启动子应用于植物基因工程的研究和产业化。
The promoter can drive the heat induced expression of foreign gene in transgenic plant and may be used to replace 35s promoter applied in plant genetic engineering research and industrialization.
目的:利用基因工程技术,构建表达具溶栓活性的纳豆激酶的大肠杆菌工程菌。
AIM: to construct engineered E. coli strains which can express nattokinase with fibrinolysis activity using gene engineering technology.
结论成功克隆人胰腺组织激肽原酶基因,并高效表达融合蛋白,为进一步开发基因工程药物打下基础。
CONCLUSION the fusion protein of the cloned kininogenase gene was highly expressed in E. coli and could be used for the development of its biological products.
目的:优化重组铜绿假单胞菌外毒素A(PEA)基因工程菌的发酵条件,实现PEA的高效表达。
Objective:To optimize the fermentable conditions of recombinant E. coli BL21 for high level expression of PEA.
同时,培养细胞实验说明不同细胞的感 染和表达的特性不同。以上结果可以为利用家蚕生产生物药品的基因工程提供参考。
Our primary observation results may provide valuable data for development of the bio-engineering using the silkworm to produce protein medicine for human beings.
同时,培养细胞实验说明不同细胞的感 染和表达的特性不同。以上结果可以为利用家蚕生产生物药品的基因工程提供参考。
Our primary observation results may provide valuable data for development of the bio-engineering using the silkworm to produce protein medicine for human beings.
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