拟获得在大肠杆菌中能高效可溶表达的转铁蛋白受体单链抗体与链亲和素（SA）的重组融合蛋白。
To obtain the anti-transferrin receptor scFv and streptavidin(SA) recombinant fusion proteins which were high-efficiently and solubly expressed in E.
结果：融合蛋白以可溶性形式存在于细菌裂解液的上清中，其表达量为菌体总蛋白量的7%。
RESULTS: Fusion protein existed in supernatant of the bacteria lysate and its expression level was about 7% of the total bacteria protein.
第一部分阐述了人胰岛素受体配基结合结构域L1和L2的可溶性表达并鉴定了它们的配基结合能力。
The first part of the thesis describes the expression and characterization of the human insulin receptor's ligand-binding domains L1 and L2.
本研究成功利用原核表达系统实现了对CT抗原NY-ESO-1的可溶性表达。
So human CT antigen NY-ESO-1 could be successfully expressed in prokaryotic expression system.
结果使蛋白以可溶形式表达，得到了纯度较高、抗原性较好、产生抗体滴度高的抗原蛋白。
The results showed that the bacteria protein became dissolved by changing the expressing condition, and the target protein becames very pure and could bring high level antibody to HBcAg.
结论只表达目的基因的抗原结构域，可以缩短表达片段的长度，有利于获得可溶性目的蛋白，并且具有良好的血清学反应的特异性。
The antigen regions of the aimed gene were selected to be expressed, which could benefit the expression of soluble fusion proteins, and the speciality of serological reaction is satisfied.
而靶向内质网中表达时，表达量达到总可溶蛋白的0.5%。
Expression levels of scFv antibodies reached up to 0.5% of total soluble proteins in leaves.
采用SDS - PAGE及岛津薄层扫描分析仪进行表达效率检测、蛋白可溶性的扫描分析。
SDS-PAGE and scanning monitor were used to detect expression efficiency and analyze the solubility of protein.
结果表明获得了人可溶性CD 14基因片段，为下一步进行CD 14表达及生物学活性研究奠定了基础。
Sequencing confirmed sCD14 gene fragment was obtained and laid the foundation for further research on expression and biological activities.
结论：MMP - 2表达增加可能受到源自口腔癌相关成纤维细胞可溶性因子的旁分泌介导，而MMP - 9表达可能需要口腔癌细胞与癌相关成纤维细胞的直接接触。
Conclusion: It was suggested that MMP-2 expression is increased by paracrine stimulation mediated by soluble factors originated from oral CAFs and MMP-9 expression requires direct cell-cell contact.
表达以可溶性蛋白和包涵体两种形式存在，其中主要是包涵体的形式，目的蛋白占不溶性蛋白的76.6%。
Inclusion body was the major pattern of the expression that amounted to 76.6 % of the insoluble protein.
将含突变基因的重组质粒转化大肠杆菌菌株bl2 1 (DE3)，经IPTG诱导表达获得的目的蛋白质均以可溶形式存在。
The recombinant plasmids containing mutant genes were transformed into the Escherichia coli strain BL21 (DE3), and the expressed proteins were found to be water soluble after the induction of IPTG.
细胞表面CD 40l表达及e -选择素与川崎病冠状动脉损伤有关，而CD 8 +T细胞表面CD 40l的表达及可溶性CD 40l与冠状动脉损伤无明显相关性。
In KD patients, CD40L expression on CD4 + T-cells and E-selection was correlated with the occurrence of coronary artery lesions but not CD40L expression on CD8 + T-cells and not soluble CD40L levels.
对表达蛋白的可溶性进行鉴定，结果表明表达产物GST-TE2融合蛋白以包涵体形式存在。
The solubility of GST-TE2 fusion protein was identified and the result indicated that expressed GST-TE2 protein existed as inclusion bodies.
结论原核表达的截断型人可溶性CD 4 0 L具有抑制肿瘤细胞生长作用。
Conclusion the soluble human CD40L truncate expressed in prokaryotic cells showed inhibiting effect on the growth of tumor cells.
高温条件下培养的丝状体比正常条件下培养的丝状体表达更多数量和种类的可溶性蛋白；
More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations.
获得可溶性表达的鲨肝再生因子类似物，初步研究性质与活性。
Studies on the Mechanism of Shark Hepatic Regenerative Factor on Rat Liver Regeneration;
获得可溶性表达的鲨肝再生因子类似物，初步研究性质与活性。
Studies on the Mechanism of Shark Hepatic Regenerative Factor on Rat Liver Regeneration;
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