介绍采用双光子激发荧光方法进行单分子探测的原理和自行研制的实验装置，激发光聚焦和荧光收集采用共焦方式。
We report here briefly the principle and the device of our single-molecule detection spectrometer based on two-photon excited fluorescence.
研究了不同波长的双光子激发对成像效果的影响，优化了荧光激发与探测光路，开发并完善了三维图像的恢复重建和数据分析软件。
Studied on wavelength impacts on multi-photon excitation and optimized and fixed the optical design, compiled softwares for 3D construction and data analysis.
研究了不同波长的双光子激发对成像效果的影响，优化了荧光激发与探测光路，开发并完善了三维图像的恢复重建和数据分析软件。
Studied on wavelength impacts on multi-photon excitation and optimized and fixed the optical design, compiled softwares for 3D construction and data analysis.
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