本文报告食用菌侧耳属四个种六个菌株原生质体分离与再生的试验结果。
This paper reports experimental results of protoplast preparation and regeneration with 6 strains which belong to 4 species within genus pleurotus.
研究结果表明，香菇原生质体分离诱变是一种很有应用价值的食用菌菌种选育方法。
The results indicated that protoplast formation and mutagenesis are the potential methods for strain selection and improvement in edible fungi.
对鹰嘴紫云英抗性系、半日花、霸王三种植物进行了原生质体分离和培养条件的研究。
The conditions of isolation and culture of protoplasts from resistant Astragalus cicer L. cell line, Helianthemum Songaricum Schrenk and Zygophyllum xanthoxylum were studied.
研究结果为海藻细胞及原生质体分离研究及海藻体细胞育苗的推广应用打下了良好的基础。
The results promote the studies of isolating somatic cells and protoplasts as well as the popularization of indoor artificial breeding technology of somatic cells .
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
结果表明：枣叶片分离出的原生质体活性显著高于悬浮培养物，但是产量低于悬浮培养物分离出的原生质体。
The results showed that the viability of protoplasts isolated from leaves was significantly higher than that of the suspension culture, but the yield of protoplasts was just the opposite.
原生质体是植物遗传改良的重要实验体系之一，而原生质体的分离是该体系的基础。
Protoplast is an important system for plant genetic improvement, and the isolation of protoplast is an essential step for the protoplast manipulation.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
在酶解之前对材料进行质壁分离处理使原生质体的产量提高了3.23％，存活率提高了4.2％。
Moreover, if the young leaf pieces were plasmolyzed before incubated in enzyme solution will increased the yield and activity of protoplast 3.23% and 4.2%.
原生质体融合技术是微生物育种的有效方法之一，包括原生质体制备、原生质体融合、原生质体再生及融合子的分离等主要过程。
The protoplast fusion technology is an effective ways of microbial breeding, including main processes of protoplast preparation, protoplast fusion, protoplast regeneration, fusion separation.
研究发现，分离原生质体的酶解脱壁处理可以诱导苜蓿细胞产生活性氧。
The present work revealed that the active oxygen radicals were generated from alfalfa callus cells during cell wall digestion in enzyme solution.
结果表明，质壁分离的时间、酶液浓度、渗透压及酶解时间是影响原生质体产量和质量的决定性因素。
The results showed that plasmolysis time, concentration of enzyme solutions, different osmoticum and incubation time significantly affected isolation of protoplasts from K326 leaves.
指出以叶片、愈伤组织、悬浮细胞作为分离原生质体的材料较适宜。
The isolation of protoplast, being the first step in protoplast technique, was restricted by several factors such as the resource materials, enzyme combination and osmotic condition.
用纤维素酶(按照中国科学院上海植物生理研究所的方法制备)分离酒草叶肉细胞，获得大量完整的有活力的原生质体。
Usiug the cellulase "EA_3-867" prepared according to the method of the plant Physiology Institute of China, a large amount of vital and intact protoplasts are isolated from the Tobacco variety.
分离出的原生质体形态饱满浑圆，FDA测定活力良好。
The isolated protoplasts whose sizes were plumpness and round in size and vigor were nice determined with FDA.
分离出的原生质体形态饱满浑圆，FDA测定活力良好。
The isolated protoplasts whose sizes were plumpness and round in size and vigor were nice determined with FDA.
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