这是迄今第一个由籼稻原生质体再生植株的报道。
This is the first report of plant regeneration from the protoplast culture of an indica rice.
原生质体再生菌株仍保留亲株的菌落形态及合成赤霉素的生产能力。
The yield of Gibberellin and morphology of colonies of the regenerated strains from protoplasts are the same as those of the parent strain.
结果表明，纤维二糖培养基及双层培养法比较适合灵芝原生质体再生。
The results showed that the cellobiose medium and double layer culture method were most suitable for protoplast regeneration of Ganoderma lucidum.
研究结果对建立高效的龙眼原生质体再生系统及其遗传操作有参考价值。
This study would be of value for establishing high-effective regeneration system of protoplasts and the related genetic manipulations in longan.
同时，对原生质体再生菌株的形态与多杀菌素产量变化进行了比较研究。
In addition, we compared the change of morphology and spinosyns yield of the regenerated strains.
研究结果对建立高效的烟草原生质体再生系统及其遗传操作有参考价值。
This research would be of some referential value for establishing high-effective regeneration system of protoplasts and the related genetic manipulations based on protoplasts in tobacco.
大豆因其愈伤组织难以分化、原生质体再生困难等因素，其再生体系一直不够完善。
The regeneration system of soybean is not perfect because the differentiation of callus tissue and the protoplast regeneration are difficult.
用较高渗透压的培养基培养原生质体再生的细胞团及愈伤组织，可提高植株再生频率。
Using media with relatively high osmotic pressure in cul-turing protoplast-derived calli increased their plant regeneration frequency on subsequent differentiation media.
采用麸皮再生培养基替代R2YE再生培养基，原生质体再生率、生长及筛选效果得到明显改善。
The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.
获得了粳型水稻77- 170品系原生质体再生植株(R_2)6个株系的206棵后代植株。
A total number of 206 regenerated plants (R2) of 6 plant lines of rice strain 77-170 from protoplasts were obtained.
单核原生质体再生菌丝的菌落形态、菌丝生长速度和营养缺陷型标记等性状均稳定地保持了亲本的特性。
The characters of parents, such as morphology of colony, growth rate of mycelium and auxotroph, were maintained in protoplasted monokaryon.
阿氏假囊酵母在原生质体再生过程中具有较高的突变率，以维生素B＿2产量为指标的正突变率为12．14％。
Many mutants appeared in the regeneration strains on solid medium. Positive mutation ratio was 12.14% by determining the yield of Vitamin B_2.
为提高海南霉素产量，分别对其产生菌进行氮离子束注入、原生质体再生等处理，获得高产菌株2-7、Y81。
High producing mutants 2 7 and Y81 of hainanmycin were obtained by ion beam implantation or protoplast regeneration.
原生质体融合技术是微生物育种的有效方法之一，包括原生质体制备、原生质体融合、原生质体再生及融合子的分离等主要过程。
The protoplast fusion technology is an effective ways of microbial breeding, including main processes of protoplast preparation, protoplast fusion, protoplast regeneration, fusion separation.
本文还详细研究了菌龄、酶系统、酶解温度和时间等多种因素对这两种鸡纵菌原生质体形成和再生的影响。
While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzymolysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.
在原生质体形成及再生的最佳条件下制备原生质体，结果发现，SD- 142菌的原生质体形成率及再生率分别达到了86.8%和31.3%。
Under the optimum conditions, the rates of formation and regeneration of protoplasts of stain SD-142 had reached to 86.8% and 31.3% respectively.
马铃薯叶肉细胞原生质体培养后，再生细胞形成细胞团和愈伤组织。
The mesophyll cell protoplasts of potato could divide from cell colonies and callus after cultured.
报道了新抗生素M-90产生菌原生质体的制备、再生及对产抗生素的影响。
The paper reports the formation and regeneration of protoplast from the new antibiotics, Micromonospora M 90, as well as the effects on antibiotics yield producing strain.
预分化实验表明：ABA能有效地提高原生质体愈伤组织的再生能力。
Predifferentiation showed that ABA was effective to increase the frequency of regeneration of protoplast-derived calli.
油菜已经建立了子叶、胚轴、原生质体及小孢子培养的再生体系。
Rape have established regeneration system of cotyledons, hypocotyls, protoplasts and microspore culture.
对甘薯原生质体培养植株再生、利用原生质进行体细胞杂交和作为遗传工程受体的研究进行了综述。
It is reviewed on protoplast culture plant regeneration, somatic hybridization by protoplast, and protoplast as an acceptor of genetic engineering in this literature.
芦笋的花药、花粉、茎尖及原生质体经离体培养均可再生植株。
The anther, pollen, stem point and bioplast cultured in vitro can be plant.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
对原生质体释放和再生进行了详细的形态发生学观察和描述。
The morphological process of protoplast formation and regeneration was studied also.
研究了利用珍稀食用菌茶树菇双核菌丝进行原生质体分离和再生，以及原生质体单核菌株的筛选过程。
The preparation of monokaryon protoplasts and the screening of protoplast-derived monokaryon strains are important parts in mushroom research and breeding.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
本文报告食用菌侧耳属四个种六个菌株原生质体分离与再生的试验结果。
This paper reports experimental results of protoplast preparation and regeneration with 6 strains which belong to 4 species within genus pleurotus.
目的：对柱晶白霉素产生菌SK-0 6的原生质体制备和再生的条件，以及再生菌的产生抗生素能力进行了研究。
AIM The condition of protoplast formation and regeneration of leucomycin (LM) producing strain SK-06 and the ability of antibiotic producing of regeneration strains was studied in this paper.
因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。
So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.
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