骨骼肌在制成单细胞悬液过程中的人为损伤，可造成DNA提前降解。
The artificial damage of the skeletal muscle during the preparation of single cell suspension could cause the earlier degradation of the DNA.
方法：采用流式细胞术对30例新鲜肺癌组织和5例正常对照组组织制成的单细胞悬液进行了DNA含量分析。
Methods:We analyzed the single-cell suspensions of 30 fresh lung carcinoma tissues and 5 normal tissues of control group to get their DNA levels by using flow cytometry.
结论消化振荡法对人胚胎神经干细胞的活性影响较小，是一种有效、安全的将神经球打散成单细胞悬液的实验方法。
Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里，剪成两瓣，用无菌玻璃的边缘挤压，获得单细胞悬液。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
C57BL小鼠10只，注射B16单细胞悬液于小鼠腹腔（腹腔组）和左后肢（后肢组），构建小鼠黑色素瘤生物力学模型；
The biomechanical animal model was built by injecting the B16F10 melanoma cell suspension into the abdominal cavity and left skeletal muscles in 10 C57BL mice.
本文对食管癌组织、脱落细胞和血液标本的悬液制备方法作了初步探讨，结果表明，胶原酶对实体肿瘤组织单细胞悬液制备是可行的。
In this report, the method for preparing mono-cellular suspensions from solid mass of esophagus carcinoma, exfoliative cells and blood sample was described.
本文对食管癌组织、脱落细胞和血液标本的悬液制备方法作了初步探讨，结果表明，胶原酶对实体肿瘤组织单细胞悬液制备是可行的。
In this report, the method for preparing mono-cellular suspensions from solid mass of esophagus carcinoma, exfoliative cells and blood sample was described.
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