方法用半巢式聚合酶链反应方法检测72例非甲—庚型肝炎患者血清中ttv - DNA。
Methods TTV-DNA in serum of 72 patients with hepatitis Non-A to G were detected by Hn-PCR.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
结论本试验建立的半巢式PCR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。
Compared with the conventional PCR, semi-nested PCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
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