方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
方法：在表达及分离纯化VEGF的基础上，成功地制备了VEGF抗体，以夹心法测定vegf。
Methods: Based on isolation, purification and expression of VEGF, successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF.
纯化的抗体不能直接用于其它已知抗原的检测，它是从天然兔血浆中分离出来的，通过蛋白A亲和色谱法制备。
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
目的：建立一条从孕早期妇女血清中分离纯化早孕因子(EPF)的技术路线，获得高纯度的EPF，并对制备抗epf单克隆抗体进行阶段性的初步尝试。
Object: Set up a method of isolation and purification of early pregnancy factor (EPF) from human pregnancy sera and get pure EPF, then attempt to prepare monoclonal antibodies.
在先前的研究中，CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
在先前的研究中，CP的分离主要依赖抗体、液相色谱以及亲合标签纯化等技术。
Typically, in previous reports, the purification of CP mainly relied on the antibody, liquid chromatography and affinity tag-based strategies.
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