摘 要：酶免疫标记、胶体金免疫标记及荧光免疫标记技术是目前应用最广泛的免疫反应检测技术。
Enzyme immuno-labeling assay, gold nanoparticle immuno-labeling and fluorescence immuno-labeling assay are used most widely in immunoassay.
本文以藻红蛋白为荧光探针，探讨了藻红蛋白荧光标记技术及荧光探针在烟草花叶病毒免疫检测中的应用效果。
The fluorescence labeling technique of phycoerythrin and application for the immunoassay of Tobacco mosaic Virus were studied in this research using it as a labeling probe.
用HRP逆行标记技术和免疫细胞化学双重标记法结合切断实验印证了NG内神经元向NTS投射的纤维性质。
HRP retrograde transport and PAP double labelling method combined with nodose ganglionectomy to detect nature of projecting fibers from the NG to the NTS.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
方法：免疫细胞化学双重标记技术。
Methods: The double labeling immunocytochemical staining technique was used.
方法采用免疫细胞化学双重标记技术。
Methods Double labeling immunocytochemical staining technique was used.
此外，结合免疫胶体金标记技术在免疫检测与环境检测实际工作中的应用，展望了其在堆肥环境检测中的应用潜力和发展前景。
In addition, the new applications and prospects of immunogold labeling technique in compost detection were proposed based on the applications in immunoassay and environmental analysis.
方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
采用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）和免疫组化方法检测鼻咽低分化鳞状细胞癌和正常鼻咽黏膜组织。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique and immunohistochemistry method were applied to detect NPC tissue and normal nasopharyngeal tissue (NNPT).
方法采用免疫细胞化学双重标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
方法：用激光共聚焦双重免疫荧光标记技术检测PLP在分化成熟的两型星形胶质细胞和少突胶质细胞中的表达情况。
Methods: Laser confocal double immunofluorescence staining method was used to detect the expression of PLP in the mature type-1 astrocytes, type-2 astrocytes and oligodendrocytes.
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