传代培养细胞可在1 ~2周汇合成片。
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
方法：原位传代培养羊水细胞并制备染色体，G显带分析核型，产后随访。
Methods:Amniotic fluid cells were cultured in situ and their karyotypes were analyzed by G band, followed them after delivery.
结果原代培养的细胞ALP染色阳性区域可以达90%以上，传代培养后细胞阳性率为100%。
Results the area positive for ALP staining is no less than 90% in the primary culture and almost 100% in the subculture.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
自成年新西兰兔股骨转子部取得骨髓基质细胞进行传代培养，所得骨髓基质细胞再与钙磷陶瓷载体复合培养制成复合人工骨。
Bone marrow cells from New Zealand white rabbits were cultured to obtain stromal cells, which were then cultured with the carriers to make the artificial composite bone graft.
取大鼠肱三头肌，采用二步消化法分离骨骼肌卫星细胞，体外进行原代培养和传代培养。
Rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
结论人羊膜上皮细胞在体外可成功进行原代和传代培养，体外培养的人羊膜上皮细胞在一定时间内可维持增殖能力。
Conclusion Human amniotic epithelial cells can be successfully primary cultured and passaged in vitro, and the cells can proliferate for a certain period.
结果流式细胞术证明，MSC具有间充质细胞的特征。原代及传代培养显示，胎儿骨髓msc具有活跃增殖的能力。
Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
目的：研究甲肝病毒在细胞内快速连续传代培养病毒增殖情况，探索疫苗生产工艺改进的可能性。
Objective: to study the proliferation of HAV which were cultured rapidly and consecutively in cells, and to investigate the possibility of improving the production process of inactivated HAV vaccine.
方法利用手术显微镜及显微手术器械分离大鼠肠系膜淋巴管。采用植块培养法进行内皮细胞原代培养并传代培养。
Methods With an operating microscope and microsurgical instruments, rat's mesenteric lymphatics were isolated and the endothelium were primarily cultured and subcultured.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
方法对着色性干皮病患者成纤维细胞进行传代培养、建株，采用放射自显影和细胞融合技术分析X P 细胞互补组。
Methods From four XP patients, skin fibroblast cell strains were derived and used for the complementation group analysis by autoradiography and cell fusion technique.
细胞传代培养和冻存6个月，7td1MC 5细胞对IL - 6的反应性和依赖性均无明显变化。
No obvious variation of reactivity and dependence of 7td1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.
结果：原代培养的成纤维细胞特征典型，并通过传代培养得到了纯化的成纤维细胞。
Results: The cultured cells showed typical shape and features of fibroblast cell, which were successfully dissociated and purified.
通过组织块培养法，获得了传代培养的鲁西黄牛耳皮肤成纤维细胞和上皮细胞。
Luxi cattle-ear skin fibroblast and epithelial cells were derived by fragment of tissue.
方法大鼠胸主动脉平滑肌细胞传代培养，实验共分六组。
Methods Rat aortic smooth muscle cells were divided into 6 groups randomly.
传代培养口腔鳞癌细胞株，制备肿瘤细胞条件培养液；
Oral squamous cells carcinoma cells lines were cultured and passaged, the conditional medium of tumor cells were prepared.
考察不同血清对山羊成纤维细胞传代培养的影响。
The effect of different types of serum on the passage of the goat fibroblasts was investigated in this work.
结论软骨细胞可以通过传代培养获得扩增，但仅限于四代以内细胞。
Conclusions RCC cells can be amplified by passage Culture, but only within the cells of four generations.
通过制备大鼠含药血清，将人源乳腺癌细胞传代培养后，分别加入药物血清常规培养，采用CCK-8试剂盒检测其细胞存活率；
The human breast cancer cells were cultured with rat serum containing either the diluent of drug or in combination or CTX. The cancer cell vitality was detected using a CCK-8 kit.
目的：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
AIM: the key of the human embryonic stem cell culture is to guarantee the totipotency and inhibit spontaneous differentiation.
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
硫酸氨基葡萄糖可以抑制这一趋势，促进传代培养的细胞增殖能力。
GS can inhibit the tendency and strengthened the proliferating ability of chondrocytes in serial subcultivation.
贴壁细胞传代培养10代后接近融合，细胞扩增了约10 0 0倍。
The adherent cells approached confluence after 10 passages, and was increased in number about 1000-fold.
方法：用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。
Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro.
方法：采用酶消化法传代培养乳sd大鼠颅盖骨细胞并建立缺氧模型。
Methods: the osteoblasts obtained from the calvaria of neonatal SD rats were cultured by method of enzyme digestion in vitro.
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