这在本质上与亲和层析是一样的。
经几丁质亲和层析柱纯化21肽。
The soluble 21 peptide was purified by using chitin affinity chromatography.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白A和肽段亲和层析纯化得到。
Antibodies are purified by protein A and peptide affinity chramotography.
抗体由蛋白a和肽段亲和层析纯化得到。
Antibodies are purified by protein a and peptide affinity chromatography.
抗体由蛋白a和肽段亲和层析技术纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
亲和层析分别纯化腹水和血清得到单克隆抗体和多克隆抗体。
The monoclonal antibody and polyclonal antibody were respectively purified from ascites and serum by affinity chromatography.
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
IPTG诱导该基因的表达，亲和层析和原位裂解法纯化表达产物。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
亲和层析是基于生物分子间特异性的亲和作用而建立的分离纯化技术。
Affinicty chromatography is a separating and purifying technique based on the specific affinity between biomolecules.
该抗体通过使用合成肽段免疫动物而获得，并经蛋白a和肽亲和层析纯化。
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein a and peptide affinity chromatography.
目的研究肝型碱性磷酸酶n -糖链亲和层析在原发性肝癌诊断中的应用价值。
Objective to study the applicatory value of affinity chromatography of N-linked sugar Chain of the liver-type alkaline phosphatase in diagnosis of primary hepatocarcinoma.
经超声波破碎菌体释放目蛋白，并用GST亲和层析进一步纯化得到目的蛋白。
The target proteins were released from the cells by sonication and further purified with GST column.
提纯的包涵体纯度可达70%以上，用镍亲和层析方法纯化蛋白则可达到95%。
The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
在非变性条件下，用TALON金属亲和层析树脂纯化含组氨酸标记的重组DHFR。
The recombinant DHFR protein containing his-tag was purified by using TALON metal affinity resin under nondenaturing conditions.
本研究报道一种基于固定化金属亲和层析(IMAC)的噬菌体抗体库液相筛选方法。
In this study, a new method based on immobilized metal affinity chromatography (IMAC) for screening phage antibody library against antigen in solution was reported.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91%。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
结论染料配基亲和层析可作为面包酵母乙醇脱氢酶和葡萄糖-6-磷酸脱氢酶的精制纯化方法。
Conclusion Dye affinity chromatography can be used as a refine purification method for purification of EDH and G-6-PDH from baker's yeast.
方法取正常小鼠肝脏，裂解肝脏后利用磷酸盐金属亲和层析(PMAC)树脂提取磷酸化蛋白。
Methods Phosphoproteins were extracted from lysates of normal mice livers by phosphate metal affinity chromatography (PMAC) resin.
本文采用亲和层析法纯化的兔抗64DP抗体，建立了简便灵敏的酶免疫测定法（ELISA）。
We have established a rapid and sensitive method-ELISA for quantitative assay of 64DP. 24 samples were parellelly examined by ELISA and rocket electrophoresis.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
结论用尼龙滤膜zbm改进的膜亲和层析法，可用于纯化腹水中的单克隆抗体，且简便、省时、有效。
Conclusion The modified MAC with ZBM is a much easier, time-saving and effective method for affinity purification of antibodies in ascites.
包涵体蛋白经含8m尿素的结合缓冲液溶解后，用金属亲和层析法进行纯化，获得了高纯度的目的蛋白。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
根据亲和层析的原理，把血红素作为配基制备亲和层析柱用于纯化HBP，为进一步研究HBP打下基础。
So in this study, based on the mechanism of affinity chromatography, the affinity chromatography column with the heme immobilized was built to do purification of HBP.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
利用DEAE离子交换和atp亲和层析法从热休克的大鼠肝脏中分离纯化了热休克蛋白70 (HSP70)。
Heat shock protein 70 (HSP70) was purified from rat liver with DEAE ion exchange chromatography and ATP-affinity chromatography.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
多克隆抗体由合成化肽段免疫动物，该合成肽段与YAP人源蛋白序列一致，抗体由蛋白a和肽段亲和层析技术纯化。
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the human sequence of YAP. Antibodies are purified by protein a and peptide affinity chromatography.
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