本试验以甲型副伤寒沙门氏菌体抗原多克隆抗体的制备及提纯为例,提出可行的更简易的提纯方法。
In this experiment, we took the example of preparation and purification of polyclonal antibody of somatic antigen of Salmonella paratyphi a and found out a simpler feasible way of purification.
结论制备了具有高亲和性和特异性的3d聚合酶多克隆抗体,为3d聚合酶的生物学功能和抗原表位研究奠定了基础。
This polyclonal antibody may lay a foundation for the further studies on the biological functions and epitopes of the 3d polymerase of FMDV.
结论:原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
结论:原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
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