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They spiked droplets of human mucus with live flu virus, and then exposed it to air with varying levels of moisture.
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Earlier, the commission slapped a ban on all imports of live birds and untreated feathers from both Turkey and Romania, where bird flu was also detected.
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In addition, people who live with or care for those at high risk for complications from the flu, including healthcare workers, and household visitors of persons at high risk for complications from the flu should be vaccinated.
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Sales of live chicken in key markets in eastern China have been suspended to contain the spread H7N9 flu.
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The two types of vaccine that have been approved -- a flu shot made from inactivated or dead virus and a nasal spray made from live, weakened virus -- will be available free of charge, though some providers may charge an "administration fee, " Sebelius said.
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Live poultry markets in Shanghai that were shut on April 6 in a move to control the spread of the flu will remain closed, the newspaper said.
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