碱性磷酸酶标记的羊抗鼠二抗和显色试剂盒购自华美公司。
The goat - anti -mouse second antibody was bought from Huamei Biological Company.
碱性磷酸酶标记的羊抗兔二抗购自北京中山生物技术有限公司。
Alkaline phosphatase conjugated goat anti - rabbit antibody was purchased from Beijing ZhongShan Biotechnology Company.
本发明也公开了用该细胞系制备单克隆抗体、抗体提纯方法及该抗体的辣根过氧化酶标记法。
The invention also discloses preparation of the monoclonal antibody by using the cell line, a method for purifying the antibody and a horseradish peroxidase labeling method for the antibody.
用酶标记免疫吸附测定法测定大鼠血浆VEGF水平，免疫组化方法测定肺组织vegf表达。
Plasma VEGF level was measured with ELISA method, and VEGF expression in lung was detected with immunohistochemistry method.
我们认为酶标记抗原直接elisa法为HFRS的早期诊断提供了更为特异、简便快速的新方法。
We think that direct ELISA that USES hrp-labeled antigen provides a more specific, simpler and faster method for early diagnosis of HFRS.
方法用SPA法在载体上固定系列浓度酶标记抗体，绘制抗体浓度-吸光度曲线，寻找SPA与抗体的最佳结合条件。
Methods Used SPA method to immobilize the enzyme conjugated antibody of different concentrations! and drew the antibody-OD line.
酶免疫检测(EIA)技术，是根据抗原抗体反应具有高度的特异性，将酶标记物的抗体作为标准试剂来鉴定未知的抗原。
Based on high specificity of antigen-antibody reaction, enzyme immunoassay (EIA) identified unknown antigen by antibody labelled with enzyme as standard reagent.
在实验中采用EHF病毒抗原与酶标记物混合后一次加入，而不是分别依次加入的方式，使操作步骤简化，实验时间缩短，又适当提高了试验敏感性。
In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously.
方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
方法：用噻唑蓝（MTT）法、光镜及电镜、DNA凝胶电泳和原位DNA末端转移酶标记法，分别对耐药细胞株BIU-87／ADM及敏感细胞株BIU-87细胞进行了阿霉素诱导的细胞凋亡研究。
Methods: MTT assay, optical microscopy, electron microscopy, DNA agarose gel electrophoresis and in situ DNA nick end labeling were used to detect the apoptosis in BIU87/ADM and BIU87 cells.
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
由于很少有健康的细胞制造这些酶，所以它们是一种可靠的肿瘤标记物。
Few healthy cells make these enzymes, so they are a reliable marker of tumours.
该研究小组还利用新的探针开发了一种新的蛋白标记方法，称为探针的酶介导法(PRIME)。
The group also developed a new protein labeling method, called probe incorporation mediated by enzymes (PRIME), which utilizes the new probes.
一系列的方法已被用来探讨这个问题，其中一个最常用的方法是研究女性肿瘤，她们是X连锁标记酶杂合子。
A variety of approaches have been used to address this issue, the most common of which is the study of tumors in women, who are heterozygous for X-linked marker enzymes.
肿瘤标记物通常是阴性。肝脏酶在正常和显著升高之间。
Tumor markers are generally negative. Liver enzymes range from normal to markedly elevated.
最近的一些研究表明环氧化酶2 (COX - 2)的表达可以作为发现乳腺癌细胞的标记。
Recent studies implicated expression of cyclooxygenase 2 (COX-2) as a marker to identify precursor cells for breast cancer.
限制性酶切片段长度多态性（RFLP）作为共显性的遗传标记，已广泛应用于遗传病的产前诊断。
As a codominant genetic marker, restriction fragment length polymorphisms, referred as RFLPs, has been widely Applied to the prenatal diagnosis of some genetic diseases.
初步显示高效液相色谱蛋白酶解肽谱分析中，荧光检测硫代磷酸化荧光标记肽的可能性。
Therefore, the possibility to detect the thiophosphorylated and fluorophore labeled peptide during high performance liquid chromatography peptide mapping was primarily shown.
在一步层析检测中，不需要额外程序(如酶反应)来产生信号的标记是首选的。
Labels that do not require additional procedures (e. g., enzyme reactions) for signal generation are preferred in one-step chromatographic assay.
方法应用细胞培养、疫组织化学、荧光标记、光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
由于形态学、细胞学、同工酶、RFLP等标记技术在大麦基因组中的多态性较低，限制了其应用。
Its applications were limited for the low polymorphism in the gene group of barley using the labeling technique of morphology, cytology, isoenzyme and RFLP.
本研究采用地高辛标记的RNA探针，用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶产生细胞的个体发育。
In this study, the ontogeny of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization using a digoxigenin-labeled RNA probe.
需要使用包括聚合酶链反应（PCR），琼脂糖凝胶电泳，探针的标记与检测等生物工程技术。
This technique employs standard operations of biomedical engineering, such as polymerase chain reaction(PCR), agarose gel electrophoresis, labeling and detecting of probe.
用脱氧核糖核酸末端转移酶介导的缺口末端标记技术（TUNEL）原位检测细胞凋亡。
Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique were applied to deterimine the apoptotic cells in all the tissues mentioned above.
其他细胞器标记酶活性在质膜组分中很低或没有。
Activitiesof marker enzyme for other organelles are low or not detectable in PMfraction.
糖类分解代谢酶基因作为安全标记基因，近年来在植物转化中显示了巨大应用潜力。
The genes coding enzymes that catalyze special sugars have showed big practical potency in plant transformation as safe marker genes.
糖类分解代谢酶基因作为安全标记基因，近年来在植物转化中显示了巨大应用潜力。
The genes coding enzymes that catalyze special sugars have showed big practical potency in plant transformation as safe marker genes.
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