研究分析了O型口蹄疫病毒(FMDV)结构蛋白vp1与当前猪f MDV疫苗血清的免疫反应性。
In this study, the immunoreactivity of structural protein VP1 of foot-and-mouth disease virus (FMDV) type o with sera from swine vaccinated against FMDV was analyzed.
目的:在体外检测口蹄疫病毒融合表位基因的表达。
Objective: to detect the expression of the fusion epitopes gene of foot-and-mouth disease virus (FMDV) in vitro.
建立了一种同时检测猪口蹄疫病毒(FMDV)、猪水泡病病毒(SVDV)和猪水疱性口炎病毒(VSV)三种病原体的多重rt - PCR方法。
A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV).
采用RT—PCR方法对口蹄疫病毒O/NY00株基因组L片段进行了分子克隆和序列测定。
The L fragment of the genome of foot-and-mouth disease virus(FMDV) O/NY00 isolate was cloned by RT-PCR, and sequenced.
目的为了克服灭活口蹄疫病毒疫苗可能存在的传播病毒的潜在危险,构建一种能预防O型口蹄疫病毒感染的VP1表位重组蛋白疫苗。
Objective to developed a recombinant protein vaccine with epitopes on VP1 protein for prevention of foot-and-mouth disease virus (FMDV) spreading by inactive-FMD vaccine.
用表达的口蹄疫3a蛋白作为包被抗原,建立了间接elisa试验,对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。
An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.
旨在建立一种检测口蹄疫病毒非结构蛋白抗体的敏感、特异的ELISA方法。
To develop a sensitive and specific ELISA for detection of antibodies to the nonstructural protein of FMDV.
采用RT-PCR方法对口蹄疫病毒O/LZ株基因组序列进行了分子克隆和序列测定。
The genome of Foot-and-Mouth disease virus(FMDV) O/LZ isolate was cloned by RT-PCR, and sequenced.
能与口蹄疫病毒感染血清发生特异性反应,而不能与疫苗免疫动物血清发生反应;
The purified protein could specificially react with FMDV infection antibodies in Western blotting assay, but no reaction with the immune antibodies induced with vaccine.
口蹄疫病毒是小RNA病毒科口蹄疫病毒属的唯一成员,不含囊膜的病毒粒子包含有一个单链正股RNA基因组。
FMDV is a member of the picornavirus family. Non-enveloped FMDV has a single stranded positive-sense RNA genome.
口蹄疫病毒是小RNA病毒科口蹄疫病毒属的唯一成员,不含囊膜的病毒粒子包含有一个单链正股RNA基因组。
FMDV is a member of the picornavirus family. Non-enveloped FMDV has a single stranded positive-sense RNA genome.
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